[三羰基甲基环戊二烯基锰（MMT）对大鼠肝线粒体的影响。I. 体外对氧化磷酸化的影响（作者译）]

[Effects of methylcyclopentadienyl manganese tricarbonyl (MMT) of rat liver mitochondria. I. Effects, in vitro, on the oxidative phosphorylation (author's transl)].

作者信息

Autissier N, Dumas P, Brosseau J, Loireau A

出版信息

Toxicology. 1977 Feb;7(1):115-22. doi: 10.1016/0300-483x(77)90043-9.

Abstract

Methylcyclopentadienyl manganese tricarbonyl (MMT) is an effective inhibitor of mitochondrial respiration associated with NAD+-linked substrates. At concentrations which inhibit glutamate-malate oxidation (over 80% inhibition) succinate and tetramethylphenylenediamine (TMPD)-ascorbate are inhibited less than 25%. MMT inhibits both electron and energy transfer in mitochondria as revealed by the partial release of MMT inhibition by 2,4-dinitrophenol (DNP). The data indicated that the effects of MMT are supported by energy conservation at site I of the respiratory chain.

摘要

甲基环戊二烯基三羰基锰（MMT）是一种与NAD⁺相关底物有关的线粒体呼吸有效抑制剂。在抑制谷氨酸 - 苹果酸氧化的浓度下（抑制率超过80%），琥珀酸和四甲基对苯二胺（TMPD） - 抗坏血酸的抑制率小于25%。如2,4 - 二硝基苯酚（DNP）部分解除MMT抑制所显示的那样，MMT抑制线粒体中的电子传递和能量转移。数据表明，MMT的作用得到呼吸链位点I处能量守恒的支持。