Morse A C, Erwin V G, Jones B C
Program in Biobehavioral Health, College of Health and Human Development, Pennsylvania State University, University Park 16802.
Pharmacol Biochem Behav. 1993 Aug;45(4):905-12. doi: 10.1016/0091-3057(93)90138-j.
We recently conducted an experiment to investigate the possible cooperation between genetic makeup and differential housing on cocaine self-administration in male and female C57BL/6J and DBA/2J mice. Cocaine self-selection was measured in a two-choice test with one choice being cocaine-HCl solution of 40 mg% in tap water and the other choice being plain tap water. Housing conditions began at weaning (21-23 days of age) and consisted of group housed (GH) with 2-3 mice per cage, and isolated housed (IH) with 1 mouse per cage. The results of this study revealed overall strain, sex and housing differences, with C57BL/6Js consuming more cocaine solution than DBA/2J subjects, females consuming more cocaine solution than males, and group housed consuming more than isolate housed subjects. In a second study, the effect of differential housing on open-field locomotor activity was investigated. Testing was conducted on two consecutive days, with subjects receiving an IP injection of saline on day 1, and 15 mg/kg cocaine HCl on day 2. Four behaviors were recorded, including: total distance, nosepokes, stereotypy, and margin time. Overall, the results revealed significant strain differences for stereotypy and nosepokes, and males were found to be more activated by cocaine than females. Additionally, DBA males tended to be differentially affected by housing condition, with IH showing suppressed locomotor activity as compared to GH subjects. Last, significant strain by housing interactions occurred in nosepokes and stereotypy time.
我们最近进行了一项实验，以研究基因组成与不同饲养方式对雄性和雌性C57BL/6J及DBA/2J小鼠可卡因自我给药行为的潜在协同作用。在一项二选一测试中测量可卡因自我选择情况，一种选择是自来水中40mg%的盐酸可卡因溶液，另一种选择是普通自来水。饲养条件从断奶期（21 - 23日龄）开始，包括每笼饲养2 - 3只小鼠的群居饲养（GH）方式，以及每笼饲养1只小鼠的单独饲养（IH）方式。本研究结果揭示了品系、性别和饲养方式的总体差异，C57BL/6J小鼠比DBA/2J小鼠消耗更多可卡因溶液，雌性比雄性消耗更多可卡因溶液，群居饲养的小鼠比单独饲养的小鼠消耗更多。在第二项研究中，调查了不同饲养方式对旷场运动活动的影响。测试连续进行两天，第1天给实验对象腹腔注射生理盐水，第2天注射15mg/kg盐酸可卡因。记录了四种行为，包括：总路程、鼻触次数、刻板行为和边缘时间。总体而言，结果显示刻板行为和鼻触次数存在显著的品系差异，且发现雄性比雌性对可卡因更敏感。此外，DBA雄性小鼠受饲养条件的影响存在差异，与群居饲养的小鼠相比，单独饲养的小鼠运动活动受到抑制。最后，鼻触次数和刻板行为时间出现了显著的品系与饲养方式的交互作用。
