Yun M H, Choi S J, Shim S C
Department of Chemistry, Korea Advanced Institute of Science and Technology, Yusong-gu, Taejon, Korea.
Photochem Photobiol. 1993 Aug;58(2):164-8. doi: 10.1111/j.1751-1097.1993.tb09543.x.
Irradiation of thin films consisting of 4,5',8-trimethylpsoralen (TMP), adenosine and small amounts of alcohols led to TMP-alcohol photoadducts in addition to TMP-adenosine photoadducts. Four TMP-ethanol and two TMP-methanol adducts have been separated and characterized. Covalent bonds were formed between the 4-carbon of TMP and the alpha-carbon to the hydroxy group in the alcohols. The TMP-alcohol photoadducts were formed only in the TMP film containing small amounts of alcohol and adenosine. Furthermore, no photoadduct of TMP and ribose was detected upon photolysis of a TMP-ribose film, suggesting that the adenine moiety plays a specific role in the reaction. The interaction of adenosine with psoralens in a dry film may be related to the DNA sequence selectivity observed for the photoreaction of psoralens with DNA.
由4,5',8-三甲基补骨脂素（TMP）、腺苷和少量醇类组成的薄膜经辐照后，除了形成TMP-腺苷光加合物外，还生成了TMP-醇光加合物。已分离并表征了四种TMP-乙醇加合物和两种TMP-甲醇加合物。TMP的4-碳与醇类中羟基的α-碳之间形成了共价键。TMP-醇光加合物仅在含有少量醇类和腺苷的TMP薄膜中形成。此外，TMP-核糖薄膜光解时未检测到TMP与核糖的光加合物，这表明腺嘌呤部分在该反应中起特定作用。干膜中腺苷与补骨脂素的相互作用可能与补骨脂素与DNA光反应中观察到的DNA序列选择性有关。
