Rutberg S E, Ronai Z
Molecular Carcinogenesis Program, American Health Foundation, Valhalla, NY 10595.
Photochem Photobiol. 1993 Aug;58(2):265-9. doi: 10.1111/j.1751-1097.1993.tb09559.x.
We have previously demonstrated the ability of UVC (254 nm) radiation to induce asynchronous polyoma replication in rat fibroblast cells (H3 line) that contain an integrated copy of polyoma virus. In the present study we show that general purpose lamps can induce polyoma replication in these cells as well. The amount of UV radiation emitted by three different light sources was determined and the effects of each source on the replication of polyoma DNA was assessed. Our findings indicate that a 100 W incandescent lamp had a minimal effect on replication, whereas a 90 s exposure to a halogen lamp or a 160 W mercury vapor lamp induced replication 1.5-fold and 2-fold, respectively, in comparison with nontreated controls. We have previously shown that asynchronous polyoma replication in H3 cells involves UV-inducible cellular protein factors. Our present results indicate that these factors are also activated by exposure to commonly used lamps that emit comparable doses of UV radiation.
我们之前已经证明，紫外线C（254纳米）辐射能够在含有多瘤病毒整合拷贝的大鼠成纤维细胞（H3系）中诱导多瘤病毒进行异步复制。在本研究中，我们表明普通灯具也能在这些细胞中诱导多瘤病毒复制。我们测定了三种不同光源发出的紫外线辐射量，并评估了每种光源对多瘤病毒DNA复制的影响。我们的研究结果表明，100瓦白炽灯对复制的影响最小，而与未处理的对照相比，暴露于卤素灯90秒或160瓦汞蒸气灯160秒分别诱导复制增加了1.5倍和2倍。我们之前已经表明，H3细胞中的异步多瘤病毒复制涉及紫外线诱导的细胞蛋白因子。我们目前的结果表明，暴露于发出相当剂量紫外线辐射的常用灯具也能激活这些因子。
