Bosiljanoff P, Porschen W, Piepenbring W, Mühlensiepen H, Feinendegen L E
Strahlentherapie. 1977 Mar;153(3):178-89.
The rate of cellular losses of hypoxic respectively euoxic cells of the solid experimental tumor "sarcoma 180" was examined in vivo after irradiations with gamma rays, 15 MeV neutrons, and alpha particles. The tumor cells were labeled in vivo, at first with 125I-UdR and after 50 hours with 131I-UdR. After a further interval of about 20 hours the tumor was irradiated. This method of double labeling makes it possible to determine externally the rates of cellular losses in the labeled zones of tumor cells presenting different partial oxygen pressures. The increase of the rates of cellular losses among the euoxic and hypoxic tumor cells induced by the gamma radiation differs by 2.6; it decreases, however, to 1.5 after injection of nitrofurazone prior to the irradiation. After irradiation with 15 MeV neutrons, a difference of only 1.4 was observed. If the tumors were irradiated internally with alpha particles from the reaction 10B(n,alpha) 7Li, there was no difference between the two rates of cellular losses. As far as the above mentioned kinds of radiation are concerned, the ratios from the increase of the rates of cellular losses induced by radiation are well corresponding to the oxygen enhancement found by other authors during their examinations in vitro.
在用γ射线、15兆电子伏中子和α粒子照射后，在体内检测实体实验肿瘤“肉瘤180”的缺氧细胞和常氧细胞的细胞损失率。肿瘤细胞在体内首先用125I - UdR标记，50小时后用131I - UdR标记。在大约20小时的进一步间隔后，对肿瘤进行照射。这种双重标记方法使得能够从外部确定呈现不同局部氧压的肿瘤细胞标记区域中的细胞损失率。γ辐射诱导的常氧和缺氧肿瘤细胞中的细胞损失率增加相差2.6；然而，在照射前注射呋喃西林后，该差值降至1.5。在用15兆电子伏中子照射后，仅观察到1.4的差值。如果用10B(n,α)7Li反应产生的α粒子对肿瘤进行内部照射，则两种细胞损失率之间没有差异。就上述几种辐射而言，辐射诱导的细胞损失率增加的比值与其他作者在体外检查期间发现的氧增强情况非常吻合。
