• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

一种对铜绿假单胞菌外毒素A具有抗性的突变型CHO-K1细胞系无法加工新城疫病毒的前体融合糖蛋白。

A mutant CHO-K1 strain with resistance to Pseudomonas exotoxin A is unable to process the precursor fusion glycoprotein of Newcastle disease virus.

作者信息

Inocencio N M, Moehring J M, Moehring T J

机构信息

Department of Microbiology and Molecular Genetics, Markey Center for Molecular Genetics, University of Vermont, Burlington 05405-0068.

出版信息

J Virol. 1993 Jan;67(1):593-5. doi: 10.1128/JVI.67.1.593-595.1993.

DOI:10.1128/JVI.67.1.593-595.1993
PMID:8416387
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC237401/
Abstract

RPE.40, a mutant strain of CHO-K1 cells isolated for resistance to Pseudomonas exotoxin A and cross-resistant to alphaviruses, is also highly resistant to virulent strains of Newcastle disease virus. The resistance of RPE.40 cells to Newcastle disease virus results from the failure to cleave the viral envelope precursor glycoprotein Fo to fusion glycoprotein F1 at the consensus sequence (Lys/Arg)-Arg-Gln-(Lys/Arg)-Arg.

摘要

RPE.40是一种从对铜绿假单胞菌外毒素A具有抗性且对甲病毒具有交叉抗性的CHO-K1细胞中分离得到的突变株，它对新城疫病毒的强毒株也具有高度抗性。RPE.40细胞对新城疫病毒的抗性源于无法在共有序列（赖氨酸/精氨酸）-精氨酸-谷氨酰胺-（赖氨酸/精氨酸）-精氨酸处将病毒包膜前体糖蛋白Fo裂解为融合糖蛋白F1。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7a5a/237401/d3681e677907/jvirol00022-0618-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7a5a/237401/d3681e677907/jvirol00022-0618-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7a5a/237401/d3681e677907/jvirol00022-0618-a.jpg

相似文献

1
A mutant CHO-K1 strain with resistance to Pseudomonas exotoxin A is unable to process the precursor fusion glycoprotein of Newcastle disease virus.一种对铜绿假单胞菌外毒素A具有抗性的突变型CHO-K1细胞系无法加工新城疫病毒的前体融合糖蛋白。
J Virol. 1993 Jan;67(1):593-5. doi: 10.1128/JVI.67.1.593-595.1993.
2
A mutant CHO-K1 strain with resistance to Pseudomonas exotoxin A and alphaviruses fails to cleave Sindbis virus glycoprotein PE2.一种对铜绿假单胞菌外毒素A和甲病毒具有抗性的突变型CHO-K1细胞系无法切割辛德毕斯病毒糖蛋白PE2。
J Virol. 1991 May;65(5):2332-9. doi: 10.1128/JVI.65.5.2332-2339.1991.
3
Expression of mouse furin in a Chinese hamster cell resistant to Pseudomonas exotoxin A and viruses complements the genetic lesion.小鼠弗林蛋白酶在中国仓鼠细胞中表达，该细胞对铜绿假单胞菌外毒素A和病毒具有抗性，可弥补遗传缺陷。
J Biol Chem. 1993 Feb 5;268(4):2590-4.
4
Selection of Pseudomonas exotoxin-resistant cells with altered expression of alpha 2MR/LRP.选择具有α2MR/LRP表达改变的抗绿脓杆菌外毒素细胞。
Ann N Y Acad Sci. 1994 Sep 10;737:138-44. doi: 10.1111/j.1749-6632.1994.tb44307.x.
5
Strains of CHO-K1 cells resistant to Pseudomonas exotoxin A and cross-resistant to diphtheria toxin and viruses.对铜绿假单胞菌外毒素A具有抗性且对白喉毒素和病毒具有交叉抗性的CHO-K1细胞系。
Infect Immun. 1983 Sep;41(3):998-1009. doi: 10.1128/iai.41.3.998-1009.1983.
6
Structural comparison of the cleavage-activation site of the fusion glycoprotein between virulent and avirulent strains of Newcastle disease virus.新城疫病毒强毒株与无毒株融合糖蛋白裂解激活位点的结构比较
Virology. 1987 May;158(1):242-7. doi: 10.1016/0042-6822(87)90261-3.
7
Characterization of the sites of proteolytic activation of Newcastle disease virus membrane glycoprotein precursors.
J Biol Chem. 1988 Sep 5;263(25):12522-31.
8
Quantitative basic residue requirements in the cleavage-activation site of the fusion glycoprotein as a determinant of virulence for Newcastle disease virus.新城疫病毒融合糖蛋白裂解激活位点的定量碱性残基需求作为毒力的决定因素
J Virol. 1988 Jan;62(1):354-6. doi: 10.1128/JVI.62.1.354-356.1988.
9
A host range mutant of Newcastle disease virus with an altered cleavage site for proteolytic activation of the F protein.一种新城疫病毒的宿主范围突变体，其F蛋白蛋白水解激活的裂解位点发生了改变。
Virus Res. 1990 Mar;15(3):237-42. doi: 10.1016/0168-1702(90)90031-6.
10
Characterization of Newcastle disease virus envelope glycoproteins expressed in insect cells.在昆虫细胞中表达的新城疫病毒包膜糖蛋白的特性分析。
Virus Res. 1994 Aug;33(2):123-37. doi: 10.1016/0168-1702(94)90049-3.

引用本文的文献

1
Bovine parainfluenza virus type 3 (BPIV3) fusion and hemagglutinin-neuraminidase glycoproteins make an important contribution to the restricted replication of BPIV3 in primates.牛副流感病毒3型（BPIV3）融合糖蛋白和血凝素-神经氨酸酶糖蛋白对BPIV3在灵长类动物中的限制性复制起重要作用。
J Virol. 2000 Oct;74(19):8922-9. doi: 10.1128/jvi.74.19.8922-8929.2000.
2
Characterization of a Chinese hamster ovary cell line developed by retroviral insertional mutagenesis that is resistant to Sindbis virus infection.通过逆转录病毒插入诱变开发的对辛德毕斯病毒感染具有抗性的中国仓鼠卵巢细胞系的特性分析。
J Virol. 1999 Jun;73(6):4919-24. doi: 10.1128/JVI.73.6.4919-4924.1999.
3

本文引用的文献

1
Nucleotide sequence of the 26S mRNA of Sindbis virus and deduced sequence of the encoded virus structural proteins.辛德毕斯病毒26S mRNA的核苷酸序列及编码的病毒结构蛋白的推导序列。
Proc Natl Acad Sci U S A. 1981 Apr;78(4):2062-6. doi: 10.1073/pnas.78.4.2062.
2
Strains of CHO-K1 cells resistant to Pseudomonas exotoxin A and cross-resistant to diphtheria toxin and viruses.对铜绿假单胞菌外毒素A具有抗性且对白喉毒素和病毒具有交叉抗性的CHO-K1细胞系。
Infect Immun. 1983 Sep;41(3):998-1009. doi: 10.1128/iai.41.3.998-1009.1983.
3
Structural comparison of the cleavage-activation site of the fusion glycoprotein between virulent and avirulent strains of Newcastle disease virus.
Endoprotease PACE4 is Ca2+-dependent and temperature-sensitive and can partly rescue the phenotype of a furin-deficient cell strain.
内切蛋白酶PACE4是钙离子依赖性且对温度敏感的，并且能够部分挽救弗林蛋白酶缺陷细胞株的表型。
Biochem J. 1999 May 1;339 ( Pt 3)(Pt 3):639-47.
4
Furin: a mammalian subtilisin/Kex2p-like endoprotease involved in processing of a wide variety of precursor proteins.弗林蛋白酶：一种哺乳动物枯草杆菌蛋白酶/Kex2p样内切蛋白酶，参与多种前体蛋白的加工。
Biochem J. 1997 Nov 1;327 ( Pt 3)(Pt 3):625-35. doi: 10.1042/bj3270625.
5
A furin-defective cell line is able to process correctly the gp160 of human immunodeficiency virus type 1.一种弗林蛋白酶缺陷型细胞系能够正确加工1型人类免疫缺陷病毒的糖蛋白160。
J Virol. 1994 Jun;68(6):4075-9. doi: 10.1128/JVI.68.6.4075-4079.1994.
6
Lethality of PE2 incorporation into Sindbis virus can be suppressed by second-site mutations in E3 and E2.E3和E2中的第二位点突变可抑制PE2掺入辛德毕斯病毒的致死性。
J Virol. 1994 Apr;68(4):2683-92. doi: 10.1128/JVI.68.4.2683-2692.1994.
7
Isolation and characterization of a novel mutant mouse cell line resistant to Newcastle disease virus: constitutive interferon production and enhanced interferon sensitivity.一种对新城疫病毒具有抗性的新型突变小鼠细胞系的分离与鉴定：组成型干扰素产生及增强的干扰素敏感性
Arch Virol. 1994;139(3-4):337-50. doi: 10.1007/BF01310796.
新城疫病毒强毒株与无毒株融合糖蛋白裂解激活位点的结构比较
Virology. 1987 May;158(1):242-7. doi: 10.1016/0042-6822(87)90261-3.
4
Quantitative basic residue requirements in the cleavage-activation site of the fusion glycoprotein as a determinant of virulence for Newcastle disease virus.新城疫病毒融合糖蛋白裂解激活位点的定量碱性残基需求作为毒力的决定因素
J Virol. 1988 Jan;62(1):354-6. doi: 10.1128/JVI.62.1.354-356.1988.
5
Structure, function, and intracellular processing of paramyxovirus membrane proteins.副粘病毒膜蛋白的结构、功能及细胞内加工过程
Virus Res. 1988 May;10(2-3):113-35. doi: 10.1016/0168-1702(88)90010-x.
6
Newcastle disease virus evolution. II. Lack of gene recombination in generating virulent and avirulent strains.新城疫病毒的进化。II. 在产生强毒株和无毒株过程中不存在基因重组。
Virology. 1989 Apr;169(2):273-82. doi: 10.1016/0042-6822(89)90152-9.
7
Molecular biology of Newcastle disease virus.新城疫病毒的分子生物学
Prog Vet Microbiol Immunol. 1989;5:16-64.
8
Antigenic mapping and functional analysis of the F protein of Newcastle disease virus using monoclonal antibodies.
Arch Virol. 1986;90(1-2):97-110. doi: 10.1007/BF01314148.
9
An endoprotease homologous to the blood clotting factor X as a determinant of viral tropism in chick embryo.一种与凝血因子X同源的内蛋白酶作为鸡胚中病毒嗜性的决定因素。
EMBO J. 1990 Dec;9(12):4189-95. doi: 10.1002/j.1460-2075.1990.tb07643.x.
10
Mammalian subtilisins: the long-sought dibasic processing endoproteases.哺乳动物枯草杆菌蛋白酶：长期寻找的双碱性加工内切蛋白酶。
Cell. 1991 Jul 12;66(1):1-3. doi: 10.1016/0092-8674(91)90129-m.