辛德毕斯病毒26S mRNA的核苷酸序列及编码的病毒结构蛋白的推导序列。
Nucleotide sequence of the 26S mRNA of Sindbis virus and deduced sequence of the encoded virus structural proteins.
作者信息
Rice C M, Strauss J H
出版信息
Proc Natl Acad Sci U S A. 1981 Apr;78(4):2062-6. doi: 10.1073/pnas.78.4.2062.
Abstract
The nucleotide sequence of intracellular 26S mRNA of Sindbis virus has been determined by direct sequence analysis of the cDNA made to this RNA with reverse transcriptase. From this, the amino acid sequences of the encoded virus structural proteins, which include a basic capsid protein and two integral membrane glycoproteins, have been deduced. The features of these proteins as related to their functions are discussed. We suggest that three proteases are required to produce these proteins from their polyprotein precursor: a viral protease, which functions in the cytosol to release the capsid protein, signalase, which makes two cleavages to separate the glycoproteins; and a protease of the Golgi complex that cleaves after double basic residues, to process the precursor form of one of the glycoproteins.
摘要
通过用逆转录酶对辛德毕斯病毒细胞内26S mRNA的RNA进行直接序列分析，已确定了其核苷酸序列。由此推导出了编码的病毒结构蛋白的氨基酸序列，这些结构蛋白包括一种碱性衣壳蛋白和两种整合膜糖蛋白。讨论了这些蛋白质与其功能相关的特征。我们认为从其多蛋白前体产生这些蛋白质需要三种蛋白酶：一种病毒蛋白酶，其在细胞质中起作用以释放衣壳蛋白；信号肽酶，其进行两次切割以分离糖蛋白；以及高尔基体复合物中的一种蛋白酶，其在双碱性残基后进行切割，以加工其中一种糖蛋白的前体形式。
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