Carson D D, Tang J P, Julian J
Department of Biochemistry and Molecular Biology, University of Texas M. D. Anderson Cancer Center, Houston 77030.
Dev Biol. 1993 Jan;155(1):97-106. doi: 10.1006/dbio.1993.1010.
Cell surface expression of the basement membrane form of heparan sulfate proteoglycan (HSPG), perlecan, basic fibroblast growth factor (bFGF) binding forms of heparan sulfate, and laminin has been examined by immunofluorescence during the attachment and outgrowth phases of mouse embryo development in vitro and in utero. The results indicate that both HSPG and laminin are expressed at the exterior surface of the trophectoderm during the attachment phase of implantation in vivo. HSPG expression correlated with acquisition of attachment competence by embryos in vitro. The intensity of immunofluorescence signal appeared to decrease by the outgrowth stage of development in vitro. It is concluded that expression of HSPG at the trophectodermal surface is coordinated with development of attachment competence by mouse embryos in vitro and in utero.
在体外和子宫内小鼠胚胎发育的附着和生长阶段，通过免疫荧光法检测了硫酸乙酰肝素蛋白聚糖（HSPG）的基底膜形式、基底膜聚糖、硫酸乙酰肝素的碱性成纤维细胞生长因子（bFGF）结合形式以及层粘连蛋白的细胞表面表达。结果表明，在体内植入的附着阶段，HSPG和层粘连蛋白均在滋养外胚层的外表面表达。HSPG表达与体外胚胎附着能力的获得相关。在体外发育的生长阶段，免疫荧光信号强度似乎降低。结论是，滋养外胚层表面HSPG的表达与体外和子宫内小鼠胚胎附着能力的发育相协调。
