Fujimoto K, Yasue H, Nakao K, Yamamoto H, Hitoshi Y, Jougasaki M, Okumura K, Ogawa H, Takatsu K, Miyamoto E
Division of Cardiology, Kumamoto University School of Medicine, Japan.
J Histochem Cytochem. 1993 Jan;41(1):35-42. doi: 10.1177/41.1.8417110.
To investigate the developmental, physiological and pathophysiological roles of human cardiac myosin light-chain 1 (LC1s), we developed two novel monoclonal antibodies (KA1 and KB1) against human cardiac LC1s and examined LC1s in normal and pathological hearts immunohistochemically. KA1 and KB1 were specific only for atrial LC1 (ALC1) and for both ALC1 and ventricular LC1 (VLC1), respectively, in human hearts. Among human tissues tested, including skeletal muscle, vascular smooth muscle, and liver, KA1 did not crossreact with proteins in any other tissues than atria, whereas KB1 crossreacted with the slow-type LC1 of skeletal muscle. Among adult mammalian hearts of several other species including pig, dog, hamster, and rat, KA1 and KB1 crossreacted only with ALC1 and with both ALC1 and VLC1, respectively. ALC1 was strongly and uniformly observed in human fetal atria and ventricles and in normal adult human atria, but sporadically in normal adult human ventricles. In the overloaded ventricle (dilated cardiomyopathy), ALC1 was highly augmented but not uniform. These results suggest that the fetal VLC1 is immunohistochemically identical to the adult type of ALC1 and that ALC1 is expressed homogeneously in human fetal ventricles and sporadically in normal adult ventricles, and is re-expressed heterogeneously and in an increased amount in the overloaded ventricle.
为了研究人心脏肌球蛋白轻链1（LC1s）的发育、生理和病理生理作用，我们研发了两种针对人心脏LC1s的新型单克隆抗体（KA1和KB1），并通过免疫组织化学方法检测了正常和病理心脏中的LC1s。在人心脏中，KA1仅对心房LC1（ALC1）具有特异性，而KB1分别对ALC1和心室LC1（VLC1）都具有特异性。在所检测的人体组织中，包括骨骼肌、血管平滑肌和肝脏，KA1除了与心房中的蛋白质发生交叉反应外，与其他任何组织中的蛋白质均无交叉反应，而KB1与骨骼肌的慢型LC交叉反应。在包括猪、狗、仓鼠和大鼠在内的其他几种成年哺乳动物心脏中，KA1和KB分别仅与ALC1以及与ALC1和VLC1发生交叉反应。在人胎儿的心房和心室以及正常成年人的心房中均强烈且均匀地观察到ALC1，但在正常成年人的心室中则偶尔出现。在负荷过重的心室（扩张型心肌病）中，ALC1高度增加但并不均匀。这些结果表明，胎儿VLC1在免疫组织化学上与成年型ALC1相同，并且ALC1在人胎儿心室中均匀表达，在正常成年心室中偶尔表达，而在负荷过重的心室中则异质性地重新表达且表达量增加。
