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REB1位点是酿酒酵母中RNA聚合酶I终止子的一个重要组成部分。

The REB1 site is an essential component of a terminator for RNA polymerase I in Saccharomyces cerevisiae.

作者信息

Lang W H, Reeder R H

机构信息

Hutchinson Cancer Research Center, Seattle, Washington 98104.

出版信息

Mol Cell Biol. 1993 Jan;13(1):649-58. doi: 10.1128/mcb.13.1.649-658.1993.

DOI:10.1128/mcb.13.1.649-658.1993
PMID:8417359
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC358943/
Abstract

We have identified a terminator for transcription by RNA polymerase I in the genes coding for rRNA of the yeast Saccharomyces cerevisiae. The terminator is located 108 bp downstream of the 3' end of the mature 25S rRNA and shares several characteristics with previously studied polymerase I terminators in the vertebrates. For example, the yeast terminator is orientation dependent, is inhibited by its own sequence, and forms RNA 3' ends 17 +/- 2 bp upstream of an essential protein binding site. The recognition sequence for binding of the previously cloned REB1 protein (Q. Ju, B. E. Morrow, and J. R. Warner, Mol. Cell. Biol. 10:5226-5234, 1990) is an essential component of the terminator. In addition, the efficiency of termination depends upon sequence context extending at least 12 bp upstream of the REB1 site.

摘要

我们已经在酿酒酵母编码核糖体RNA的基因中鉴定出一种RNA聚合酶I转录的终止子。该终止子位于成熟25S rRNA 3'端下游108 bp处，与先前在脊椎动物中研究的聚合酶I终止子具有几个共同特征。例如，酵母终止子具有方向依赖性，受其自身序列抑制，并在一个必需的蛋白质结合位点上游17 +/- 2 bp处形成RNA 3'末端。先前克隆的REB1蛋白（Q. Ju、B. E. Morrow和J. R. Warner，《分子与细胞生物学》10:5226 - 5234，1990）结合的识别序列是终止子的一个重要组成部分。此外，终止效率取决于至少在REB1位点上游延伸12 bp的序列背景。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1317/358943/3f2950a80d64/molcellb00013-0681-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1317/358943/1e928c533cfa/molcellb00013-0676-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1317/358943/bde6afe8f3c6/molcellb00013-0676-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1317/358943/8c573ba22671/molcellb00013-0677-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1317/358943/25e34a39b671/molcellb00013-0678-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1317/358943/07a980026d1d/molcellb00013-0679-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1317/358943/fb66fc47c082/molcellb00013-0679-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1317/358943/32bf10e60697/molcellb00013-0680-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1317/358943/7687ca761348/molcellb00013-0680-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1317/358943/721742d951b2/molcellb00013-0681-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1317/358943/3f2950a80d64/molcellb00013-0681-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1317/358943/1e928c533cfa/molcellb00013-0676-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1317/358943/bde6afe8f3c6/molcellb00013-0676-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1317/358943/8c573ba22671/molcellb00013-0677-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1317/358943/25e34a39b671/molcellb00013-0678-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1317/358943/07a980026d1d/molcellb00013-0679-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1317/358943/fb66fc47c082/molcellb00013-0679-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1317/358943/32bf10e60697/molcellb00013-0680-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1317/358943/7687ca761348/molcellb00013-0680-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1317/358943/721742d951b2/molcellb00013-0681-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1317/358943/3f2950a80d64/molcellb00013-0681-b.jpg

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