Herbert D C, Yashiro T, Muraki T, Okano T, Hattori A, Suzuki T
Department of Cellular and Structural Biology, University of Texas Health Science Center, San Antonio 78284-7762.
Anat Rec. 1993 Jan;235(1):121-5. doi: 10.1002/ar.1092350112.
A quantitative analysis of the pituitary gland was conducted to ascertain the effects of protein-calorie malnutrition on the morphology of the somatotrophs, gonadotrophs, thyrotrophs, and corticotrophs. Male rats were fed a low, 8% protein diet from 20 to 50 days of age, while their age-matched controls were given a diet containing 27% protein. The hypophyses were then processed for light microscopic immunocytochemical staining using antibodies to growth hormone, the beta subunits of luteinizing hormone and thyroid stimulating hormone, and adrenal corticotrophic hormone. The number of each cell type along with an evaluation of the cell, cytoplasmic, and nuclear areas was conducted using a computerized image analyzer. All of these parameters were reduced significantly in the somatotrophs as a result of the low protein diet, while in the gonadotrophs, the cell, cytoplasmic, and nuclear areas were similarly affected. Smaller cell number, cell area, and nuclear area were noted in the corticotrophs of the malnourished animals, while in the thyrotrophs, only the cell and nuclear areas were reduced. The data demonstrate that each pituitary cell type responds in a unique manner to undernutrition.
对垂体进行了定量分析，以确定蛋白质 - 热量营养不良对生长激素细胞、促性腺激素细胞、促甲状腺激素细胞和促肾上腺皮质激素细胞形态的影响。雄性大鼠在20至50日龄时喂食低蛋白（8%）饮食，而年龄匹配的对照组则给予含27%蛋白质的饮食。然后使用针对生长激素、促黄体生成素和促甲状腺激素的β亚基以及促肾上腺皮质激素的抗体，对垂体进行光镜免疫细胞化学染色处理。使用计算机图像分析仪对每种细胞类型的数量以及细胞、细胞质和细胞核面积进行评估。由于低蛋白饮食，生长激素细胞的所有这些参数均显著降低，而促性腺激素细胞的细胞、细胞质和细胞核面积也受到类似影响。营养不良动物的促肾上腺皮质激素细胞数量、细胞面积和细胞核面积较小，而促甲状腺激素细胞中，只有细胞和细胞核面积减小。数据表明，每种垂体细胞类型对营养不足的反应方式独特。
