Klaasen H L, Van der Heijden P J, Stok W, Poelma F G, Koopman J P, Van den Brink M E, Bakker M H, Eling W M, Beynen A C
Central Animal Laboratory, Catholic University, Nijmegen, The Netherlands.
Infect Immun. 1993 Jan;61(1):303-6. doi: 10.1128/iai.61.1.303-306.1993.
Segmented filamentous bacteria (SFBs) are apathogenic autochthonous bacteria in the murine small intestine that preferentially attach to Peyer's patch epithelium. SFBs have never been cultured in vitro. We have studied the effects of SFBs on the immune system of the host. Mice monoassociated with SFBs were compared with germ-free mice and with mice without SFBs but with a specific-pathogen-free (SPF) gut flora. SFBs versus no microbial flora raised the number of lymphoid cells in the lamina propria of the ileal and cecal mucosa, raised the number of immunoglobulin A (IgA)-secreting cells in the intestinal mucosa, produced elevated IgA titers in serum and intestinal secretions, and enhanced the concanavalin A-induced proliferative responses of mesenteric lymph node cells. The SPF flora had effects similar to but less pronounced than those mediated by SFBs. The results indicate that SFBs stimulate the mucosal immune system to a greater extent than do other autochthonous gut bacteria.
分节丝状菌(SFBs)是小鼠小肠中的一种非致病性本土细菌,它优先附着于派尔集合淋巴结上皮。SFBs从未在体外培养成功。我们研究了SFBs对宿主免疫系统的影响。将单定殖有SFBs的小鼠与无菌小鼠以及没有SFBs但有特定病原体-free(SPF)肠道菌群的小鼠进行比较。与无微生物菌群相比,SFBs增加了回肠和盲肠粘膜固有层中淋巴细胞的数量,增加了肠道粘膜中分泌免疫球蛋白A(IgA)的细胞数量,使血清和肠道分泌物中的IgA滴度升高,并增强了伴刀豆球蛋白A诱导的肠系膜淋巴结细胞的增殖反应。SPF菌群的作用与SFBs介导的作用相似,但程度较弱。结果表明,SFBs比其他本土肠道细菌更能刺激粘膜免疫系统。