van den Broek M F, van Bruggen M C, van de Putte L B, van den Berg W B
Department of Rheumatology, University Hospital Nijmegen, The Netherlands.
Cell Immunol. 1988 Oct 1;116(1):216-29. doi: 10.1016/0008-8749(88)90222-5.
In order to investigate the immunological mechanism of the chronic phase of streptococcal cell wall (SCW)-induced arthritis in Lewis rats, we compared the SCW-specific T cell response in arthritis-susceptible (female Lewis) and resistant (F344) rats. We present evidence that this T cell response is absent in F344 rats, while it is clearly present in Lewis rats. The T cell response was analyzed both in the spleen and in lymph nodes. In addition, we show, that injection of SCW in the F344 rat induces a general unresponsiveness in this strain: the response to mitogen was severely suppressed in SCW-injected F344 rats and, furthermore, when SCW was coinjected with ovalbumin, the response to ovalbumin was depressed. The fact that priming with ovalbumin alone induces a normal response in the F344 rat to both mitogen and ovalbumin implies that the observed abnormality after SCW priming is not a general immunological defect in this strain. Additionally, we demonstrate that adherent cells of both Lewis and F344 exert negative effects on an in vitro T cell response after injection with SCW, and that F344-adherent cells are more potent in this effect. Removal of OX8-positive cells leads to a restoration of the SCW-specific T cell response in SCW-injected F344 rats, indicating that the expression of this response is controlled by (SCW-specific?) suppressor T cells. Our results provide suggestive evidence for the obligatory role of SCW-specific T cells in the expression of chronic joint inflammation after systemic injection of SCW.
为了研究链球菌细胞壁（SCW）诱导的Lewis大鼠关节炎慢性期的免疫机制，我们比较了关节炎易感（雌性Lewis）和抗性（F344）大鼠中SCW特异性T细胞反应。我们提供的证据表明，F344大鼠中不存在这种T细胞反应，而在Lewis大鼠中则明显存在。对脾脏和淋巴结中的T细胞反应均进行了分析。此外，我们发现，向F344大鼠注射SCW会在该品系中诱导一种全身性无反应状态：在注射SCW的F344大鼠中，对丝裂原的反应受到严重抑制，而且，当SCW与卵清蛋白共同注射时，对卵清蛋白的反应也受到抑制。单独用卵清蛋白致敏能使F344大鼠对丝裂原和卵清蛋白产生正常反应，这一事实表明，SCW致敏后观察到的异常并非该品系的一般性免疫缺陷。此外，我们证明，Lewis和F344大鼠的黏附细胞在注射SCW后对体外T细胞反应均产生负面影响，且F344黏附细胞的这种作用更强。去除OX8阳性细胞可使注射SCW的F344大鼠中SCW特异性T细胞反应恢复，这表明该反应的表达受（SCW特异性？）抑制性T细胞控制。我们的结果为全身性注射SCW后慢性关节炎症表达中SCW特异性T细胞的必要作用提供了提示性证据。
