Kinne R W, Becker W, Simon G, Paganelli G, Palombo-Kinne E, Wolski A, Bloch S, Schwarz A, Wolf F, Emmrich F
Max-Planck-Society, Clinical Research Unit for Rheumatology/Immunology, Erlangen, FRG.
J Nucl Med. 1993 Jan;34(1):92-8.
Joint uptake and body distribution of a 99mTc-labeled monoclonal antibody (Mab) to the rat CD4 molecule (W3/25; IgG1) were investigated after intravenous injection in normal rats and in animals with experimentally induced adjuvant arthritis. An isotype-matched Mab with irrelevant specificity (anti-human carcino-embryonic-antigen) was used as control. A 4 hr sequential gamma-camera imaging revealed that both anti-CD4 and control Mab accumulated to a higher degree in arthritic than in normal ankle joints; the accumulation was comparable for the two Mabs. In contrast to the inflamed joints, a specific accumulation of the anti-CD4 Mab was found in organs rich in CD4-positive cells, i.e. spleen, bone marrow and lymph nodes, as assessed by direct well counter measurements 16 hr after injection. The control Mab displayed no preferential organ accumulation in either normal or diseased animals. These results indicate that a specific accumulation of anti-CD4 Mabs occurs in CD4-positive-cell-rich tissues in both normal and diseased animals and that immunoglobulins accumulate preferentially in inflamed joints regardless of their antibody specificity.
在正常大鼠和实验性诱导佐剂性关节炎动物中静脉注射99mTc标记的抗大鼠CD4分子单克隆抗体（Mab，W3/25；IgG1）后，研究了其在关节的摄取及全身分布情况。使用具有不相关特异性的同型匹配Mab（抗人癌胚抗原）作为对照。4小时的连续γ相机成像显示，抗CD4 Mab和对照Mab在关节炎踝关节中的积聚程度均高于正常踝关节；两种Mab的积聚情况相当。与炎症关节不同，注射后16小时通过直接井型计数器测量发现，抗CD4 Mab在富含CD4阳性细胞的器官（即脾脏、骨髓和淋巴结）中有特异性积聚。对照Mab在正常或患病动物中均未显示出优先的器官积聚。这些结果表明，抗CD4 Mab在正常和患病动物富含CD4阳性细胞的组织中均有特异性积聚，并且免疫球蛋白无论其抗体特异性如何，都优先积聚在炎症关节中。
