Dixon A D, Gakunga P T
School of Dentistry, University of California, Los Angeles 90024-1668.
Anat Rec. 1993 Feb;235(2):312-8. doi: 10.1002/ar.1092350215.
The proteolytic enzyme, papain, was given systematically to evaluate the short- and long-term effects of inhibition of endochondral bone formation on pelvic growth, with emphasis on the innominate bone. Ninety-eight Lewis-strain male rats, used concurrently for craniofacial growth studies, were divided into two groups. Thirty rats from Group I (n = 48) received 2% crude papain i.p. daily from 25-40 days-of-age and were euthanized at 40, 54, and 70 days-of-age. Thirty-five rats from Group II (n = 50) were given papain at the same dose from 25-70 days-of-age and were euthanized at 26, 40, 54, 70, and 120 days. The remaining animals in both groups were the controls. Standardized dorsoventral pelvic radiographs were taken of all 98 animals. Ten linear dimensions were measured on each and the data evaluated statistically. A reduction both in size and rate of growth of the bony pelvis was found. All the anteroposterior and most of the transverse pelvic dimensions were significantly shorter, to a greater extent in the prolonged papain group. Bi-ischial width was increased, perhaps to compensate for pelvic shortening and to accommodate the pelvic contents. The findings may contribute to our better understanding of abnormal endochondral bone growth in the pelvis.
系统性地给予蛋白水解酶木瓜蛋白酶，以评估抑制软骨内骨形成对骨盆生长的短期和长期影响，重点是髋骨。同时用于颅面生长研究的98只刘易斯品系雄性大鼠被分为两组。第一组（n = 48）中的30只大鼠在25至40日龄时每天腹腔注射2%的粗木瓜蛋白酶，并在40、54和70日龄时安乐死。第二组（n = 50）中的35只大鼠在25至70日龄时给予相同剂量的木瓜蛋白酶，并在26、40、54、70和120日龄时安乐死。两组中的其余动物作为对照。对所有98只动物拍摄标准化的骨盆前后位X线片。测量每张片子上的十个线性维度，并对数据进行统计学评估。发现骨盆腔的大小和生长速率均降低。所有前后径和大部分横径尺寸均明显缩短，在长期使用木瓜蛋白酶的组中缩短程度更大。坐骨结节间宽度增加，可能是为了补偿骨盆缩短并容纳盆腔内容物。这些发现可能有助于我们更好地理解骨盆中异常的软骨内骨生长。
