Kühn K, Bertling W M, Emmrich F
Max-Planck Society, Institute for Immunology, University of Erlangen-Nürnberg, Germany.
Biochem Biophys Res Commun. 1993 Jan 15;190(1):1-7. doi: 10.1006/bbrc.1993.1001.
We have identified a cDNA clone for human cytidine deaminase (EC 3.5.4.5) during an investigation which aimed at cloning novel gene expression products related to monocyte/macrophage differentiation. The derived amino acid sequence of the clone comprises 145 residues yielding a molecular mass for the polypeptide of 16.1 kDa and exhibits a nearly 50% homology to cytidine deaminase from Bacillus subtilis. Cytidine deaminase activity of the cloned sequence could be demonstrated in a prokaryotic expression system. The mRNA is highly expressed in granulocytes while expression is very low in fibroblasts, chondrocytes, monocytes, and T- as well as B-cell lines. The mRNA can be induced in monocytes, the monocytoid cell line U937 and the myeloblastic line HL 60 by the differentiation inducer calcitriol.
在一项旨在克隆与单核细胞/巨噬细胞分化相关的新型基因表达产物的研究中，我们鉴定出了人胞苷脱氨酶（EC 3.5.4.5）的一个cDNA克隆。该克隆推导的氨基酸序列包含145个残基，多肽的分子量为16.1 kDa，与枯草芽孢杆菌的胞苷脱氨酶具有近50%的同源性。克隆序列的胞苷脱氨酶活性可在原核表达系统中得到证实。该mRNA在粒细胞中高表达，而在成纤维细胞、软骨细胞、单核细胞以及T细胞和B细胞系中的表达非常低。分化诱导剂骨化三醇可诱导单核细胞、单核细胞样细胞系U937和髓母细胞系HL 60中的mRNA表达。
