Jung-Ha H, Lee Y, Kim D, Song B H
Andong University, Department of Biology, Korea.
Mol Biol Rep. 1998 Nov;25(4):205-10. doi: 10.1023/a:1006862506479.
In order to study the regulatory region for transcription, the genomic DNA of the human CDD gene was cloned and analyzed. In contrast to previously reported CDD cDNA sequence, the sequence of the isolated genomic clone was rearranged at the 5' untranslated region (UTR) by a 30 bp inversion and a 57 bp insertion. Polymerase chain reaction (PCR) of chromosomal DNA and mRNA, and sequencing analysis of the PCR products revealed that sequences corresponding to both the genomic clone and the cDNA clone were present in the chromosomal DNA and were also transcribed into mRNA. Because of the 30 base inversion, the two types of CDD transcripts contain antisense sequences in their 5' UTR. Their role in the regulation of CDD expression is discussed.
为了研究转录调控区域，对人CDD基因的基因组DNA进行了克隆和分析。与先前报道的CDD cDNA序列不同，分离的基因组克隆序列在5'非翻译区（UTR）通过30 bp的倒位和57 bp的插入发生了重排。染色体DNA和mRNA的聚合酶链反应（PCR）以及PCR产物的测序分析表明，与基因组克隆和cDNA克隆相对应的序列都存在于染色体DNA中，并且也被转录成mRNA。由于30个碱基的倒位，两种类型的CDD转录本在其5'UTR中都含有反义序列。文中讨论了它们在CDD表达调控中的作用。
