Tomilin N, Rosanov Y u, Zenin V, Bozhkov V, Vig B
Institute of Cytology of the Russian Academy of Sciences, St. Petersburg.
Biochem Biophys Res Commun. 1993 Jan 15;190(1):257-62. doi: 10.1006/bbrc.1993.1039.
We have developed a new and rapid immunofluorescent method for visualisation of replicated regions on fixed DNA fibers. Using this method we have found in 5-fluorodeoxyuridine (FrdU)-blocked human cells distinct replication units covering about 60 kb of DNA and corresponding in size to single replicons or chromatin loops. Our results also suggest that nonadjacent replicons within a replicon cluster may be activated after FrdU arrest and that the method may be adapted for localization on fibers of specific DNA sequences.
我们开发了一种新的快速免疫荧光方法，用于观察固定DNA纤维上的复制区域。使用这种方法，我们在5-氟脱氧尿苷（FrdU）阻断的人类细胞中发现了不同的复制单元，其覆盖约60 kb的DNA，大小与单个复制子或染色质环相对应。我们的结果还表明，复制子簇内不相邻的复制子在FrdU阻断后可能被激活，并且该方法可能适用于特定DNA序列在纤维上的定位。
