Mäkitie J, Teräväinen H
Acta Neuropathol. 1977 Jan 31;37(1):55-63. doi: 10.1007/BF00684541.
Nerve (NCV) and motor (MNCV) conduction velocities of the rat sciatic nerve were examined between 1 and 90 days after ischemia for 1, 2, 3, 4 or 6 h. The results were compared to light and electron microscopy of the nerve. Slight diminution in the MNCV was observed 1 day after 1-2 h ischemia, whereas 3-6 h ischemia resulted in a complete conduction block. Diminution in the NCV occurred first after ischemia for 2 h and a complete block was seen after 4 and 6 h ischemia. Reduced NCV and MNCV were seen between 4 and 18 days only in the animals subjected to ischemia of longer duration of 3-6 h. Both the NCV and MNCV were nearly normalized at the 90th day. Ischemia of 4 and 6 h resulted in denervation of some of the muscle fibers, seen as spontaneous fibrillation at the 4th and 18th day. Electron microscopy and histometric studies showed degeneration of myelinated fibers increasingly after longer durations of ischemia; ischemia for 2 h caused a degeneration of about 5%, 3 h of about 35%, 4 h about 45%, and 6 h about 75% of the fibers. Myelinated fibers of different sizes were equally damaged. In the teased fiber preparations normal and myelin sheaths undergoing Wallerian-like degeneration was seen. Regeneration occurred, but even at the 90th day there was a tendency of the myelin/axon ratio towards values less than control values.
在大鼠坐骨神经缺血1、2、3、4或6小时后的1至90天内，检测其神经传导速度（NCV）和运动神经传导速度（MNCV）。将结果与神经的光镜和电镜检查结果进行比较。缺血1至2小时后1天，观察到MNCV略有下降，而缺血3至6小时则导致完全传导阻滞。缺血2小时后首先出现NCV下降，缺血4和6小时后出现完全阻滞。仅在缺血持续时间较长（3至6小时）的动物中，在4至18天之间观察到NCV和MNCV降低。在第90天时，NCV和MNCV几乎恢复正常。缺血4和6小时导致一些肌纤维失神经支配，在第4天和第18天表现为自发纤颤。电镜和组织计量学研究显示，缺血时间越长，有髓纤维的变性越严重；缺血2小时导致约5%的纤维变性，3小时约为35%，4小时约为45%，6小时约为75%。不同大小的有髓纤维均受到同等程度的损伤。在 teased fiber 制剂中，可见正常的和正在经历类沃勒变性的髓鞘。发生了再生，但即使在第90天时，髓鞘/轴突比值仍有低于对照值的趋势。
