团藻（Volvox carteri f. nagariensis）高活性性诱导信息素在哺乳动物细胞系统中的表达

Expression of highly active sex-inducing pheromone of Volvox carteri f. nagariensis in a mammalian cell system.

作者信息

Jaenicke L, Feldwisch O, Merkl B, Cremer A, Haas I G

机构信息

Institute of Biochemistry, University of Cologne, Germany.

出版信息

FEBS Lett. 1993 Feb 1;316(3):257-60. doi: 10.1016/0014-5793(93)81303-h.

DOI:10.1016/0014-5793(93)81303-h

PMID:8422951

Abstract

A cDNA fragment coding for the sex-inducing glycoprotein of Volvox carteri f. nagariensis was expressed in a mammalian cell system (baby hamster kidney (BHK) cells). The transfection product exhibited a specific biological activity intermediate between the natural pheromone of the strains Volvox carteri f. nagariensis and Volvox carteri f. weismannia. Immunoblot analysis showed that the sex-inducing activity was expressed as a set of three iso-glycoproteins (35, 34 and 31 kDa).

摘要

编码莱茵衣藻性诱导糖蛋白的一个cDNA片段在哺乳动物细胞系统（幼仓鼠肾（BHK）细胞）中得到表达。转染产物表现出介于莱茵衣藻菌株和魏斯曼衣藻天然信息素之间的特定生物活性。免疫印迹分析表明，性诱导活性表现为一组三种同工糖蛋白（35、34和31 kDa）。