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来自嗜碱链霉菌菌株KSM-9的内切葡聚糖酶CasA是β-1,4-葡聚糖酶B家族的典型成员。

Endoglucanase CasA from alkalophilic Streptomyces strain KSM-9 is a typical member of family B of beta-1,4-glucanases.

作者信息

Damude H G, Gilkes N R, Kilburn D G, Miller R C, Warren R A

机构信息

Department of Microbiology, University of British Columbia, Vancouver, Canada.

出版信息

Gene. 1993 Jan 15;123(1):105-7. doi: 10.1016/0378-1119(93)90547-g.

Abstract

CasA is an endo-beta-1,4-glucanase from Streptomyces KSM-9 belonging to family B of beta-1,4-glucanases. A previous analysis of a portion of the corresponding gene (casA) revealed sequencing errors in a region encoding part of the catalytic site. Additional errors in the original sequence were suspected, based on sequence comparison of the C terminus of CasA with other members of its family. Re-sequencing of the remainder of the casA coding region showed that CasA is a typical member of family B.

摘要

CasA是一种来自链霉菌KSM-9的内切β-1,4-葡聚糖酶,属于β-1,4-葡聚糖酶家族B。先前对相应基因(casA)部分区域的分析揭示了编码催化位点一部分的区域存在测序错误。基于CasA C末端与家族其他成员的序列比较,怀疑原始序列中存在其他错误。对casA编码区其余部分的重新测序表明,CasA是家族B的典型成员。

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