Maki Y, Breidenbach W C, Firrell J C
Christine M. Kleinert Institute for Hand and Microsurgery, Louisville, Kentucky 40202.
J Orthop Res. 1993 Jan;11(1):20-7. doi: 10.1002/jor.1100110104.
The precision of a modified microsphere technique to measure blood flow in the hind limb of the rabbit was determined. Regional (local) injections made via a catheter in the aorta and blood withdrawn through a catheter in the hind limb enabled deposition of a high concentration of microspheres in the tissues while minimizing the number of microspheres needed for accurate measurement. 95Niobium and 141cerium-labeled 16 microns diameter microspheres were used. Blood flow was measured in 15 locations of muscle and skin for simultaneous injections into the aorta and left ventricle (group A), simultaneous injections of a mixture into the left ventricle (group B), and ventricular injections given 1 h apart (group C). No significant differences in mean blood flow measurements were found. Correlation coefficients between local and ventricular injections ranged from 0.89-0.99 and between 0.994 and 0.999 for two simultaneous ventricular injections. The variability over time was greater, with correlations between the first and second injections ranging from 0.13-0.939. Precision of the local technique compared with the ventricular technique can be kept within 30% with five or six animals. The advantage of this technique over ventricular injections is in the measurement of blood flow in small tissue samples when the number of microspheres deposited from ventricular injections is insufficient.
确定了一种改良微球技术测量兔后肢血流的精确度。通过主动脉内导管进行区域（局部）注射，并通过后肢导管抽血，可使高浓度微球沉积在组织中，同时将准确测量所需的微球数量降至最低。使用了直径为16微米的95铌和141铈标记的微球。对肌肉和皮肤的15个部位进行血流测量，测量时分别进行向主动脉和左心室同时注射（A组）、向左心室同时注射混合物（B组）以及间隔1小时进行心室注射（C组）。平均血流测量结果未发现显著差异。局部注射与心室注射之间的相关系数在0.89至0.99之间，两次同时进行的心室注射之间的相关系数在0.994至0.999之间。随时间的变异性更大，第一次和第二次注射之间的相关系数在0.13至0.939之间。与心室技术相比，使用五或六只动物时，局部技术的精确度可保持在30%以内。当心室注射沉积的微球数量不足时，该技术相对于心室注射的优势在于能够测量小组织样本中的血流。
