Lyberg T
Acta Pathol Microbiol Scand C. 1977 Feb;85(1):73-7. doi: 10.1111/j.1699-0463.1977.tb03613.x.
The effect of rabbit antisera on the uptake and release of alpha-amino-isobutyric acid (AIBA) in LS- and P-388 cells was investigated. In the presence of homologous antiserum and complement, the uptake of AIBA was inhibited. In the absence of complement, no effect of antiserum was seen. Using prelabelled cells, the efflux of AIBA was greatly accelerated in the presence of antiserum and complement. The AIBA-uptake method was compared with the trypan blue exclusion test and the 51Cr release technique. The AIBA-uptake method was more sensitive in quantitative cytotoxic studies.
研究了兔抗血清对LS-和P-388细胞摄取和释放α-氨基异丁酸(AIBA)的影响。在同源抗血清和补体存在的情况下,AIBA的摄取受到抑制。在没有补体的情况下,未观察到抗血清的作用。使用预先标记的细胞,在抗血清和补体存在的情况下,AIBA的流出大大加速。将AIBA摄取法与台盼蓝排斥试验和51Cr释放技术进行了比较。在定量细胞毒性研究中,AIBA摄取法更敏感。