Rayner B L, Williams D S, Oliver S
Renal Unit, Groote Schuur Hospital.
S Afr Med J. 1993 Jan;83(1):42-3.
The aim of the study was to determine if direct inoculation of peritoneal fluid into Bactec blood culture bottles would improve the positive bacteriological yield compared with conventional techniques in continuous ambulatory peritoneal dialysis (CAPD) patients with peritonitis. All patients presenting with suspected peritonitis had peritoneal fluid injected directly into aerobic and anaerobic Bactec blood culture bottles as well as into sterile culture tubes. Thirty-seven paired samples were analysed. Twenty conventional cultures (54%) were positive compared with 33 (89%) done according to the Bactec system (P < 0.002). In only 1 case did the former technique prove superior. Direct inoculation of peritoneal fluid into Bactec blood culture bottles is therefore superior to conventional methods and has obvious therapeutic implications.
本研究的目的是确定与传统技术相比，将腹膜液直接接种到Bactec血培养瓶中是否能提高持续性非卧床腹膜透析（CAPD）腹膜炎患者的细菌学阳性检出率。所有疑似腹膜炎的患者均将腹膜液直接注入需氧和厌氧Bactec血培养瓶以及无菌培养管中。分析了37对样本。传统培养有20例（54%）呈阳性，而根据Bactec系统进行的培养有33例（89%）呈阳性（P<0.002）。仅1例显示前一种技术更优。因此，将腹膜液直接接种到Bactec血培养瓶中优于传统方法，且具有明显的治疗意义。
