使用两种培养方法检测持续不卧床腹膜透析液中的细菌生长。
Detecting bacterial growth in continuous ambulatory peritoneal dialysis effluent using two culture methods.
机构信息
Department of Internal Medicine, Konyang University College of Medicine, Daejeon, Korea.
出版信息
Korean J Intern Med. 2010 Mar;25(1):82-5. doi: 10.3904/kjim.2010.25.1.82. Epub 2010 Feb 26.
BACKGROUND/AIMS: The aim of this study was to evaluate the peritonitis-causing bacteria detected in peritoneal fluid using a blood culture bottle in patients undergoing continuous ambulatory peritoneal dialysis (CAPD).
METHODS
One-hundred and eleven dialysates from 43 patients suspected of peritonitis related to CAPD were retrospectively evaluated between May 2000 and February 2008. In all cases, 5 to 10 mL of dialysate was inoculated into a pair of BacT/Alert blood culture bottles, and 50 mL of centrifuged dialysate was simultaneously inoculated into a solid culture media for conventional culture. The results were compared to those of the conventional culture method. Isolated microorganisms were compared between the two methods.
RESULTS
The blood culture method was positive in 78.6% (88 / 112) of dialysate specimens and the conventional culture method in 50% (56 / 112, p < 0.001).
CONCLUSIONS
The blood culture method using the BacT/Alert system is useful for culturing dialysates and improves the positive culture rate in patients with suspected peritonitis compared to the conventional culture method.
背景/目的：本研究旨在评估使用血液培养瓶检测行持续非卧床腹膜透析（CAPD）患者腹膜液中腹膜炎相关细菌。
方法
回顾性评估 2000 年 5 月至 2008 年 2 月期间 43 例疑似 CAPD 相关腹膜炎的患者的 111 份透析液。所有病例均将 5 至 10 mL 的透析液接种于一对 BacT/Alert 血培养瓶中，并同时将 50 mL 离心后的透析液接种于固体培养介质进行常规培养。将结果与常规培养方法进行比较。比较两种方法分离出的微生物。
结果
血液培养法在 112 份透析液标本中的阳性率为 78.6%（88/112），而常规培养法的阳性率为 50%（56/112，p<0.001）。
结论
使用 BacT/Alert 系统的血液培养法可用于培养透析液，与常规培养法相比，可提高疑似腹膜炎患者的阳性培养率。
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