源自固有层淋巴细胞的猪白细胞介素4 cDNA的核苷酸及推导的氨基酸序列。
Nucleotide and deduced amino acid sequence of porcine interleukin 4 cDNA derived from lamina propria lymphocytes.
作者信息
Bailey M, Perry A C, Bland P W, Stokes C R, Hall L
机构信息
Department of Veterinary Medicine, University of Bristol, UK.
出版信息
Biochim Biophys Acta. 1993 Jan 23;1171(3):328-30. doi: 10.1016/0167-4781(93)90077-q.
PMID:8424960
Abstract
Total RNA was isolated from in vitro activated lamina propria lymphocytes and used to direct the synthesis of cDNA. Interleukin 4 transcripts were then specifically amplified by PCR. Comparison of the nucleotide sequence with its human homologue demonstrates deletion within the coding region of pig interleukin 4 centred around amino acid residue 70 in the mature human protein.
摘要
从体外活化的固有层淋巴细胞中分离出总RNA，并用于指导合成cDNA。然后通过PCR特异性扩增白细胞介素4转录本。将该核苷酸序列与其人类同源物进行比较，结果显示猪白细胞介素4的编码区域内存在缺失，该缺失以成熟人类蛋白质中的氨基酸残基70为中心。
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