一种与小鼠B细胞刺激因子1同源的人白细胞介素cDNA克隆的分离与鉴定，该克隆表达B细胞和T细胞刺激活性。
Isolation and characterization of a human interleukin cDNA clone, homologous to mouse B-cell stimulatory factor 1, that expresses B-cell- and T-cell-stimulating activities.
作者信息
Yokota T, Otsuka T, Mosmann T, Banchereau J, DeFrance T, Blanchard D, De Vries J E, Lee F, Arai K
出版信息
Proc Natl Acad Sci U S A. 1986 Aug;83(16):5894-8. doi: 10.1073/pnas.83.16.5894.
A cDNA sequence coding for a human interleukin has been isolated from a concanavalin A-activated human T-cell cDNA library based on homology with a mouse interleukin cDNA that expresses B-cell stimulatory factor 1 (BSF-1) activity and T-cell- and mast-cell-stimulating activities. The human cDNA contains a single open reading frame encoding a protein of 153 amino acid residues including a putative signal peptide. Amino acid sequences of the mouse and human polypeptides, deduced from their cDNAs, share extensive homology with the exception of about 40 amino acid residues near the middle portion, which share little homology. Supernatant of COS-7 monkey cells transfected with the human cDNA clone stimulated proliferation of human helper T-cell clones and of anti-IgM-activated human B cells, two properties of mouse BSF-1 on mouse cells. These results indicate that this human cDNA clone encodes a protein structurally and functionally homologous to mouse BSF-1.
基于与表达B细胞刺激因子1（BSF-1）活性以及T细胞和肥大细胞刺激活性的小鼠白细胞介素cDNA的同源性，从伴刀豆球蛋白A激活的人T细胞cDNA文库中分离出了编码人白细胞介素的cDNA序列。该人cDNA包含一个单一的开放阅读框，编码一个由153个氨基酸残基组成的蛋白质，其中包括一个推定的信号肽。从小鼠和人cDNA推导的多肽氨基酸序列具有广泛的同源性，但中间部分附近约40个氨基酸残基几乎没有同源性。用人cDNA克隆转染的COS-7猴细胞的上清液刺激了人辅助性T细胞克隆和抗IgM激活的人B细胞的增殖，这是小鼠BSF-1在小鼠细胞上的两个特性。这些结果表明，该人cDNA克隆编码一种在结构和功能上与小鼠BSF-1同源的蛋白质。
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