Hand P H, Robbins P F, Salgaller M L, Poole D J, Schlom J
Laboratory of Tumor Immunology and Biology, National Cancer Institute, National Institutes of Health, Bethesda, MD 20892.
Cancer Immunol Immunother. 1993;36(2):65-75. doi: 10.1007/BF01754404.
The MC-38 C57BL/6 mouse colon adenocarcinoma cell line has been transduced with a retroviral construct containing cDNA encoding the human carcinoembryonic antigen (CEA) gene [Robbins PF, Kantor JA, Salgaller M, Horan Hand P, Fernsten PD, Schlom J (1991) Cancer Res 51: 3657]. Two clones, MC-38-ceal and MC-38-cea2, expressed high levels of CEA on their cell surface. A third CEA-expressing cell line, MCA-102-cea3, was similarly derived by transduction of the MCA-102 C57BL/6 mouse fibrosarcoma cell line and is described here. In this study, the three CEA-transduced murine tumor cell lines (MC-38-ceal, MC-38-cea2, MCA-102-cea3) were evaluated for their tumorigenic potential, as well as their ability to serve as in vivo model systems for active and passive immunotherapy studies. Parameters that were investigated include tumor growth rate, the antibody response of the host to CEA, and the CEA content of the tumors. The MC-38-cea2 model appeared to be the most appropriate for immunotherapy studies. Biodistribution studies, using an 125I-labeled anti-CEA mAb, demonstrated efficient tumor targeting of MC-38-cea2 tumors in C57BL/6 and athymic mice.
MC-38 C57BL/6小鼠结肠腺癌细胞系已用一种逆转录病毒构建体进行转导，该构建体包含编码人癌胚抗原（CEA）基因的cDNA[罗宾斯PF，坎托JA，萨尔加勒M，霍兰·汉德P，费恩斯滕PD，施洛姆J（1991年）《癌症研究》51: 3657]。两个克隆，MC-38-ceal和MC-38-cea2，在其细胞表面高水平表达CEA。第三个表达CEA的细胞系，MCA-102-cea3，是通过转导MCA-102 C57BL/6小鼠纤维肉瘤细胞系类似获得的，在此进行描述。在本研究中，评估了三种转导CEA的小鼠肿瘤细胞系（MC-38-ceal、MC-38-cea2、MCA-102-cea3）的致瘤潜力，以及它们作为主动和被动免疫治疗研究体内模型系统的能力。研究的参数包括肿瘤生长速率、宿主对CEA的抗体反应以及肿瘤的CEA含量。MC-38-cea2模型似乎最适合免疫治疗研究。使用125I标记的抗CEA单克隆抗体进行的生物分布研究表明，MC-38-cea2肿瘤在C57BL/6和无胸腺小鼠中能有效靶向肿瘤。
