Ménard D, Arsenault P, Monfils S
Département d'Anatomie et de Biologie Cellulaire, Faculté de Médecine, Université de Sherbrooke, Québec, Canada.
Gastroenterology. 1993 Feb;104(2):492-501. doi: 10.1016/0016-5085(93)90418-c.
This investigation was undertaken to establish a serum-free organ culture technique allowing for the morphological and physiological maintenance of human fetal stomach in vitro.
Explants from gastric corpuses (12-17 weeks of gestation) were cultured in serum-free medium for periods of up to 15 days.
After 15 days of culture, surface mucous cells were more mature, gastric glands were numerous and well developed, and all epithelial cell types were morphologically very well preserved. Morphometric measurements of the glands revealed an accelerated development in culture compared with that found in utero. Even though the incorporation of [3H]thymidine into total DNA decreased, the labeling indices determined by radioautography confirmed that epithelial cell proliferation was maintained especially in the pit/neck portion and at the base of the glandular compartments. A significant increase in total glycoprotein synthesis, as evaluated by the incorporation of [3H]glucosamine, was observed and correlated with the differentiation of the mucous cells.
This investigation establishes for the first time that human gastric mucosa can be maintained up to 15 days in organ culture and that maturation of the gastric mucosa can be reproduced in chemically defined media.
开展本研究是为了建立一种无血清器官培养技术，以在体外维持人胎儿胃的形态和生理状态。
取自胃体部（妊娠12 - 17周）的外植体在无血清培养基中培养长达15天。
培养15天后，表面黏液细胞更加成熟，胃腺数量众多且发育良好，所有上皮细胞类型在形态上都保存得非常好。对腺体的形态测量显示，与子宫内相比，培养中的发育加速。尽管[3H]胸苷掺入总DNA的量减少，但放射自显影确定的标记指数证实上皮细胞增殖得以维持，尤其是在隐窝/颈部以及腺腔底部。通过[3H]葡糖胺掺入评估发现总糖蛋白合成显著增加，且与黏液细胞的分化相关。
本研究首次证实人胃黏膜在器官培养中可维持长达15天，并且胃黏膜的成熟可在化学成分明确的培养基中重现。
