Ménard D, Arsenault P
Département d'Anatomie et de Biologie Cellulaire, Faculté de Médecine, Université de Sherbrooke, Québec, Canada.
Anat Embryol (Berl). 1990;182(5):509-16. doi: 10.1007/BF00178918.
Cell proliferation during morphogenesis of human stomach was investigated using radioautography and biochemical determinations of [3H]-thymidine incorporation into DNA. Labeling indices in the epithelium, mesenchyme and muscle layer were established on radioautographs and the heights (mm) of the gastric glands were measured between 10 and 17 weeks of gestation. At 11-12 weeks, the appearance of the first pit/gland was noted, and the labeling index ranged from 9.2 to 10.2%. Labeled cells were present at all levels of the stratified epithelium. Between 14 and 16 weeks, the total epithelial labeling index declined sharply (8.1 to 5.4%) with a concomitant increase of the height of the pit/gland structures (0.055 to 0.080 mm). High proliferative activity was also recorded in the mesenchyme and the muscle layer, the labeling indices decreasing between 10 and 17 weeks. The biochemical quantitation of the [3H]-thymidine uptake into the total gastric DNA clearly supported the continuous decrease of the cell proliferation determined by radioautography. Detailed analysis of the epithelium showed that proliferative cells were more numerous at the base of the gland at the earliest stage (11 weeks) but concentrated in the pit/neck regions by 13-14 weeks. As the pit/gland development proceeded (14 to 17 weeks) labeled cells remained more abundant in the pit/neck regions of the gland (10.9%) and were rarely seen on the surface epithelium (2%). The present investigation provides basic quantitative data regarding cell proliferation in developing human stomach, and indicates that the morphogenesis of the gastric glands is correlated with the high proliferative capacity of the pit/neck cells.
利用放射自显影技术以及对掺入DNA的[3H] - 胸腺嘧啶核苷进行生化测定，研究了人胃形态发生过程中的细胞增殖情况。在放射自显影片上确定了上皮、间充质和肌肉层的标记指数，并在妊娠10至17周期间测量了胃腺的高度（毫米）。在11 - 12周时，观察到第一个胃小凹/腺体的出现，标记指数在9.2%至10.2%之间。分层上皮的各个水平均有标记细胞。在14至16周期间，上皮总标记指数急剧下降（从8.1%降至5.4%），同时胃小凹/腺体结构的高度增加（从0.055毫米增至0.080毫米）。间充质和肌肉层也记录到高增殖活性，标记指数在10至17周期间下降。对[3H] - 胸腺嘧啶核苷摄取到总胃DNA中的生化定量分析明确支持了放射自显影所确定的细胞增殖的持续下降。对上皮的详细分析表明，增殖细胞在最早阶段（11周）腺体底部较多，但到13 - 14周时集中在胃小凹/颈部区域。随着胃小凹/腺体的发育（14至17周），标记细胞在腺体的胃小凹/颈部区域仍然较多（10.9%），而在表面上皮很少见到（2%）。本研究提供了关于发育中的人胃细胞增殖的基本定量数据，并表明胃腺的形态发生与胃小凹/颈部细胞的高增殖能力相关。
