Behar J, Rhim B Y, Thompson W, Biancani P
Department of Medicine, Rhode Island Hospital, Providence.
Gastroenterology. 1993 Feb;104(2):563-8. doi: 10.1016/0016-5085(93)90427-e.
Gallbladder motility is impaired in specimens with cholesterol stones but normal with pigment stones.
Muscle cells obtained from 19 human gallbladders with cholesterol stones and 11 with pigment stones were enzymatically digested and contracted with cholecystokinin octapeptide (CCK-8), acetylcholine, and KCl.
Muscle cells from pigment stones had a greater contraction than cells from cholesterol stones. CCK-8-induced contraction was unaffected by calcium-free media but was blocked by strontium. Potassium-evoked contraction was blocked by a calcium-free media and unaffected by strontium. Inositol triphosphate (IP-3)-induced contraction was similar to the contraction caused by CCK-8 in permeable cells from pigment stones but was greater than the response to CCK-8 in cells from cholesterol stones.
Muscle cells from gallbladders with cholesterol stones contract less than cells from gallbladders with pigment stones; CCK-8-induced contraction only uses stored calcium; and IP-3 causes contractions of equal magnitude in cells from gallbladders with cholesterol and pigment stones. These abnormalities could result from an impaired receptor activation of the mechanism for IP-3 generation and release of stored calcium.
胆固醇结石标本的胆囊运动功能受损，而色素结石标本的胆囊运动功能正常。
从19例有胆固醇结石的人胆囊和11例有色素结石的人胆囊获取肌肉细胞，进行酶消化，并使用八肽胆囊收缩素（CCK-8）、乙酰胆碱和氯化钾使其收缩。
色素结石的肌肉细胞比胆固醇结石的肌肉细胞收缩更强。CCK-8诱导的收缩不受无钙培养基影响，但被锶阻断。钾诱发的收缩被无钙培养基阻断，不受锶影响。三磷酸肌醇（IP-3）诱导的收缩在色素结石可渗透细胞中与CCK-8引起的收缩相似，但在胆固醇结石细胞中大于对CCK-8的反应。
有胆固醇结石的胆囊的肌肉细胞收缩比有色素结石的胆囊的肌肉细胞少；CCK-8诱导的收缩仅利用储存的钙；IP-3在有胆固醇结石和色素结石的胆囊细胞中引起同等程度的收缩。这些异常可能是由于IP-3生成和储存钙释放机制的受体激活受损所致。
