Botarelli P, Brunetto M R, Minutello M A, Calvo P, Unutmaz D, Weiner A J, Choo Q L, Shuster J R, Kuo G, Bonino F
Department of Allergy/Immunology, Ciba-Geigy, Basel, Switzerland.
Gastroenterology. 1993 Feb;104(2):580-7. doi: 10.1016/0016-5085(93)90430-k.
To assess the role played by the immune response in the outcome of hepatitis C virus infection, the CD4+ T-lymphocyte response to viral antigens was studied in infected individuals with different clinical courses.
Using six recombinant proteins of hepatitis C virus, the study assessed the proliferative responses of peripheral blood mononuclear cells from 41 patients with chronic hepatitis C, 11 patients whose chronic hepatitis was successfully treated with interferon alfa and 11 healthy HCV seropositive individuals.
(1) Sixty-five percent of hepatitis C virus-seropositive individuals had CD4+ T-cell responses to viral proteins. (2) All viral proteins were immunogenic for T cells, although NS4 was the most immunogenic. (3) There was a significant correlation between the presence of CD4+ T cell responses to Core and a benign course of infection in healthy seropositives, most of whom were viremic.
CD4+ T-cell responses to Core, although they do not coincide with virus clearance, are associated with a benign course of infection and may be required to maintain humoral and cellular responses protective against the disease.
为评估免疫反应在丙型肝炎病毒感染转归中所起的作用，我们研究了不同临床病程的感染者对病毒抗原的CD4 + T淋巴细胞反应。
本研究采用六种丙型肝炎病毒重组蛋白，评估了41例慢性丙型肝炎患者、11例经干扰素α成功治疗的慢性肝炎患者以及11例健康HCV血清阳性个体外周血单个核细胞的增殖反应。
（1）65%的丙型肝炎病毒血清阳性个体对病毒蛋白有CD4 + T细胞反应。（2）所有病毒蛋白对T细胞均具有免疫原性，尽管NS4的免疫原性最强。（3）在健康血清阳性个体中，对核心蛋白的CD4 + T细胞反应的存在与良性感染病程之间存在显著相关性，其中大多数个体存在病毒血症。
对核心蛋白的CD4 + T细胞反应虽然与病毒清除不一致，但与良性感染病程相关，可能是维持针对该疾病的体液和细胞保护性反应所必需的。
