Junod A F, Ody C
Am J Physiol. 1977 Mar;232(3):C88-94. doi: 10.1152/ajpcell.1977.232.3.C88.
The uptake and metabolism of 5-hydroxytryptamine (5HT) and l-norepinephrine (NE) were measured in endothelial cells of pig pulmonary artery and aorta, as well as in lung slices. Evidence for oxidative deamination and for a saturable uptake mechanism, sensitive to cold, imipramine, and NA+-free medium was obtained for 5HT in lung slices and in the pulmonary as well as in the aortic endothelium, separated from the lumen by absorption to Millipore filters or by exposure to collagenase. For NE, however, a similar type of uptake could be deomonstrated in lung slices only. In the aortic and pulmonary endothelium, NE was metabolized, but its uptake was not influenced by imipramine, Na+-free medium, and high substrate concentration. The aortic and pulmonary endothelium have, therefore, similar properties and exhibit for 5HT, but not for NE, a type of uptake similar to that existing in lung capillaries.
在猪肺动脉和主动脉的内皮细胞以及肺切片中测量了5-羟色胺（5HT）和左旋去甲肾上腺素（NE）的摄取和代谢。在肺切片、肺动脉以及主动脉内皮中，通过吸附到微孔滤膜或暴露于胶原酶从管腔分离后，获得了5HT氧化脱氨和对寒冷、丙咪嗪和无钠培养基敏感的可饱和摄取机制的证据。然而，对于NE，仅在肺切片中可证明类似类型的摄取。在主动脉和肺动脉内皮中，NE被代谢，但其摄取不受丙咪嗪、无钠培养基和高底物浓度的影响。因此，主动脉和肺动脉内皮具有相似的特性，对于5HT表现出与肺毛细血管中存在的摄取类型相似，但对于NE则不然。
