Kleinzeller A, Dubyak G R, Griffin P M, McAvoy E M, Mullin J M, Rittmaster R
Am J Physiol. 1977 Mar;232(3):F227-34. doi: 10.1152/ajprenal.1977.232.3.F227.
Teased renal tubules of the winter flounder (Pseudopleuronectes americanus) were employed to investigate the structural requirements for two pathways of D-glucose transport which take place preponderantly across the basal (antiluminal) face of renal cells. 1) An inhibition analysis of the equilibrating, Na-independent and phlorizin-sensitive transport of the nonmetabolizable methyl-alpha-D-glucoside (0.1 and 0.5 mM), with 20 glucose analogs (5 mM), was employed to establish the structural requirements for the substrate-carrier interaction: a (pyranose) ring, oxygen, or F at C1, C2-OH, C3-OH, and C4-OH (all axial, 1C model). Some interaction may also occur at C6-OH. D-Glucose shares this transport system. Hydrogen bonding between the oxygens and the carrier is suggested. 2) The phloretin- and phlorizin-sensitive, ouabain-insensitive transport of D-glucose, 2-deoxy-D-glucose, and D-mannose is associated with considerable phosphorylation. The three sugars mutually compete for a shared transport site. The specificity pattern characterizing the transport system defines the following structural requirements: a (pyranose) ring, a free C1-OH, C3-OH, and C4-OH (both axial) and possibly C6-OH. Hydrogen bonding between the carrier and the oxygens at C3, C4, and C6, and covalent bonding at C1 is suggested.
利用冬季比目鱼（美洲拟庸鲽）的分离肾小管来研究D-葡萄糖转运的两条途径的结构要求，这两条途径主要发生在肾细胞的基底（抗腔）面。1）用20种葡萄糖类似物（5 mM）对不可代谢的甲基-α-D-葡萄糖苷（0.1和0.5 mM）的平衡、不依赖钠且对根皮苷敏感的转运进行抑制分析，以确定底物-载体相互作用的结构要求：一个（吡喃糖）环、氧或C1、C2-OH、C3-OH和C4-OH处的氟（均为轴向，1C模型）。C6-OH处也可能发生一些相互作用。D-葡萄糖共享这个转运系统。提示氧与载体之间存在氢键。2）D-葡萄糖、2-脱氧-D-葡萄糖和D-甘露糖对根皮素和根皮苷敏感、对哇巴因不敏感的转运与大量磷酸化有关。这三种糖相互竞争一个共享的转运位点。表征该转运系统的特异性模式定义了以下结构要求：一个（吡喃糖）环、一个游离的C1-OH、C3-OH和C4-OH（均为轴向）以及可能的C6-OH。提示载体与C3、C4和C6处的氧之间存在氢键，且C1处存在共价键。
