Pritchard J B, Kleinzeller A
Am J Physiol. 1976 Aug;231(2):603-7. doi: 10.1152/ajplegacy.1976.231.2.603.
The renal handling of several sugars was examined using clearance techniques in the winter flounder Pseudopleuronectes americanus. The nonmetabolizable sugar alpha-methyl-D-glucoside was extensively reabsorbed, with consequent accumulation in renal tissue to nearly twice plasma concentration. Both glucose and phlorizin abolished reabsorption and reduced tissue-to-plasma ratios (T/P). D-Galactose was reabsorbed. However, the T/P for free galactose was only 0.6 (total sugar was 1.7). Glucose and phlorizin produced only a transient decrease in reabsorption and no change in T/P. 2-Deoxy-D-glucose showed neither net reabsorption nore secretion. Nevertheless, kidney T/P were inexcess of 6 for total sugar and 1.2 for free sugar, indicating entry through the peritubular face of the tubule. Neither glucose nor phlorizin altered 2-deoxy-D-glucose clearance, but both reduced T/P for total sugar (2.4) and free sugar (0.7). Thus, several systems govern the handling of these sugars at the luminal membrane of the renal tubule, just as has been previously demonstrated at the peritubular membrane in this species.
利用清除技术，对美洲拟庸鲽（Pseudopleuronectes americanus）冬季个体的几种糖类肾处理情况进行了研究。不可代谢的糖类α-甲基-D-葡萄糖苷被大量重吸收，结果在肾组织中积累至血浆浓度的近两倍。葡萄糖和根皮苷均消除了重吸收并降低了组织与血浆的比率（T/P）。D-半乳糖被重吸收。然而，游离半乳糖的T/P仅为0.6（总糖为1.7）。葡萄糖和根皮苷仅使重吸收出现短暂下降，且T/P无变化。2-脱氧-D-葡萄糖既未显示出净重吸收也未显示出分泌。尽管如此，肾脏总糖的T/P超过6，游离糖的T/P为1.2，表明其通过肾小管的管周面进入。葡萄糖和根皮苷均未改变2-脱氧-D-葡萄糖的清除率，但两者均降低了总糖（2.4）和游离糖（0.7）的T/P。因此，正如之前在该物种的管周膜上所证明的那样，几种系统控制着这些糖类在肾小管管腔膜处的处理。
