Emara M G, Nestor K E, Bacon L D
Department of Poultry Science, Ohio State University, Ohio Agricultural Research and Development Center, Wooster, 44691.
Poult Sci. 1993 Jan;72(1):60-6. doi: 10.3382/ps.0720060.
A turkey subline at the Ohio Agricultural Research and Development Center was developed by DNA typing of the MHC using a chicken MHC Class II probe, and it segregated for specific MHC genotypes. Histocompatibility was examined between turkeys of known MHC genotype using skin graft procedures, mixed lymphocyte reactions (MLR), and graft-versus-host reactions (GVHR). Skin grafts were exchanged among 3-wk-old turkeys and it was found that when birds shared DNA patterns (genotypes), the skin grafts were usually accepted. In contrast, skin grafts were always rejected when birds did not share the identical DNA pattern. Similarly, MLR only occurred when the lymphocytes were derived from birds that did not share the same DNA pattern. Lastly, GVHR were examined in embryos injected with either sire or dam blood. The GVHR in embryos was dependent on the parental MHC genotype. Four MHC haplotypes were identified in the turkey subline. The turkey MHC has been designated MhcMega-B, and each of the haplotypes, Mega-B(1) through Mega-B(4).
俄亥俄农业研究与发展中心的一个火鸡亚系是通过使用鸡MHC II类探针进行MHC的DNA分型培育而成的，并且它按照特定的MHC基因型进行分离。利用皮肤移植程序、混合淋巴细胞反应（MLR）和移植物抗宿主反应（GVHR），对已知MHC基因型的火鸡之间的组织相容性进行了检测。在3周龄的火鸡之间交换皮肤移植片，结果发现，当鸟类具有相同的DNA模式（基因型）时，皮肤移植片通常会被接受。相反，当鸟类不具有相同的DNA模式时，皮肤移植片总是会被排斥。同样，只有当淋巴细胞来自不具有相同DNA模式的鸟类时，才会发生MLR。最后，对注射了父本或母本血液的胚胎进行了GVHR检测。胚胎中的GVHR取决于亲本的MHC基因型。在该火鸡亚系中鉴定出了四种MHC单倍型。火鸡MHC已被命名为MhcMega - B，并且每种单倍型分别为Mega - B(1)至Mega - B(4)。
