Yang C Y, Yang T, Yeh B K
Department of Medicine, Baylor College of Medicine, Houston, Texas.
J Protein Chem. 1993 Feb;12(1):11-4. doi: 10.1007/BF01024908.
A reliable high-performance liquid chromatography (HPLC) method is described for the separation of dimethylamino-azobenzenesulfonyl-amino acid (DABS-AA). The separation is accomplished by reversed-phase chromatography on a Hypersil ODS column (4.6 x 150 mm) with a Hewlett-Packard liquid chromatography system. In addition to the developed sample and solvent preparation procedure, this precolumn modification method using dimethylaminoazobenzene sulfonyl chloride (DABS-CL) for amino acid analysis is proved reliable and sensitive. Five apolipoprotein B-100 tryptic peptides, two of them containing cysteine, were demonstrated as examples for the general application of this method in amino acid analysis. It is a useful method for analysis of cysteine- and cystine-containing peptide and, furthermore, for determination of sulfhydryl and disulfide linkages of proteins.
描述了一种用于分离二甲基氨基偶氮苯磺酰氨基酸（DABS-AA）的可靠高效液相色谱（HPLC）方法。该分离通过在Hypersil ODS柱（4.6×150 mm）上进行反相色谱，使用惠普液相色谱系统来完成。除了所开发的样品和溶剂制备程序外，这种使用二甲基氨基偶氮苯磺酰氯（DABS-CL）进行氨基酸分析的柱前修饰方法被证明是可靠且灵敏的。作为该方法在氨基酸分析中普遍应用的示例，展示了五条载脂蛋白B-100胰蛋白酶肽段，其中两条含有半胱氨酸。它是一种用于分析含半胱氨酸和胱氨酸肽段的有用方法，此外，还可用于测定蛋白质的巯基和二硫键。
