二甲基氨基偶氮苯磺酰氨基酸的完全分离。使用二甲基氨基偶氮苯磺酰氯对低纳克量多肽进行氨基酸分析。
A complete separation of dimethylaminoazobenzenesulphonyl-amino acids. Amino acid analysis with low nanogram amounts of polypeptide with dimethylaminoazobenzenesulphonyl chloride.
作者信息
Chang J Y, Knecht R, Braun D G
出版信息
Biochem J. 1982 Jun 1;203(3):803-6. doi: 10.1042/bj2030803.
Abstract
A chromatographical system has been developed to give a complete baseline separation of all dimethylaminoazobenzenesulphonyl (DABS)-amino acids for high-sensitivity amino acid analysis [Chang, Knecht & Braun (1981) Biochem. J. 199, 547--556]. The system, which uses a Merck RP-18 column with phosphate buffer (12 mM, pH 6.5)/acetonitrile mixture, allows reliable analysis of DABS-amino acids at the 1--2 pmol level. The accuracy of this new system is demonstrated by the composition analysis of two immunoglobulin light chains (214 amino acid residues) with differences at only three amino acid residue positions.
摘要
已开发出一种色谱系统，用于在高灵敏度氨基酸分析中对所有二甲基氨基偶氮苯磺酰（DABS）-氨基酸进行完全基线分离[张、克内克特和布劳恩（1981年），《生物化学杂志》199卷，547 - 556页]。该系统使用带有磷酸盐缓冲液（12 mM，pH 6.5）/乙腈混合物的默克RP - 18柱，可对1 - 2皮摩尔水平的DABS -氨基酸进行可靠分析。通过对两条仅在三个氨基酸残基位置存在差异的免疫球蛋白轻链（214个氨基酸残基）进行组成分析，证明了该新系统的准确性。
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