人类肿瘤细胞中的微核形成：与放射敏感性缺乏相关性。

Micronucleus formation in human tumour cells: lack of correlation with radiosensitivity.

作者信息

Bush C, McMillan T J

机构信息

Radiotherapy Research Unit, Institute of Cancer Research, Sutton, Surrey, UK.

出版信息

Br J Cancer. 1993 Jan;67(1):102-6. doi: 10.1038/bjc.1993.17.

DOI:10.1038/bjc.1993.17

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC1968207/

Abstract

The micronucleus (MN) test has been carefully characterized in four human tumour cell lines of widely differing radiosensitivity. Two radioresistant bladder carcinoma cell lines (MGH-U1 and RT112), one sensitive medulloblastoma cell line (D283MED) and a sensitive neuroblastoma cell line (HX142) were used. The number of MN per Gy of ionising radiation was 0.13 for HX142, 0.17 for D283MED, 0.21 for RT112 and 0.26 for MGH-U1. This does not rank the cell lines in the same order of radiosensitivity as clonogenic cell survival where the surviving fraction at 2 Gy (SF2) was 0.11 for HX142, 0.2 for D283MED, 0.62 for RT112 and 0.53 for MGH-U1. This discrepancy between MN formation and cell death leaves doubt as to the potential usefulness of the MN test as a rapid assay of radiosensitivity but it has potential implications for the mechanistic basis of radiosensitivity in these cells.

摘要

微核（MN）试验已在四种放射敏感性差异很大的人类肿瘤细胞系中得到详细表征。使用了两种放射抗性膀胱癌细胞系（MGH-U1和RT112）、一种敏感的髓母细胞瘤细胞系（D283MED）和一种敏感的神经母细胞瘤细胞系（HX142）。每戈瑞电离辐射产生的微核数量，HX142为0.13，D283MED为0.17，RT112为0.21，MGH-U1为0.26。这并没有按照克隆形成细胞存活所确定的放射敏感性顺序对细胞系进行排序，其中2戈瑞（SF2）时的存活分数，HX142为0.11，D283MED为0.2，RT112为0.62，MGH-U1为0.53。微核形成与细胞死亡之间的这种差异，让人怀疑微核试验作为放射敏感性快速检测方法的潜在实用性，但它对这些细胞放射敏感性的机制基础具有潜在影响。

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