Ahrné S, Stenström I M, Jensen N E, Pettersson B, Uhlén M, Molin G
Department of Food Technology, University of Lund, Sweden.
Int J Syst Bacteriol. 1995 Apr;45(2):382-5. doi: 10.1099/00207713-45-2-382.
Restriction fragment length polymorphisms of the 16S rRNA genes of Erysipelothrix strains were studied by cleavage of the chromosomal DNA with restriction endonuclease EcoRI, followed by hybridization to a 420-bp internal fragment of the 16S rRNA gene. Thirty-two Erysipelothrix type and reference strains were classified, together with seven field strains. Reference strains of all serotypes and the type strains of Erysipelothrix rhusiopathiae and Erysipelothrix tonsillarum were included. Nine ribopatterns were observed. Pattern A was represented by 16 strains and included strains of serotypes 1b, 2 to 8, 11 to 13, 15 to 17, 19, and 23. Pattern B was represented by two strains (serotypes 1a and 9). Pattern C was represented by five strains (serotypes 5, 6, and 21). Pattern D was represented by one strain of serotype 4. Pattern E was represented by 11 strains of serotypes 2, 7, 10, 20, 22, 24, and 25. Patterns F, G, H, and I were each represented by a single strain of serotypes 26, 2, 18, and 3, respectively. All the different ribopatterns had some bands in common. Patterns B, C, and D were most similar to pattern A, while patterns F, G, H, and I resembled pattern E. Partial sequencing of the 16S rRNA gene of nine selected strains resulted in three different sequences, i.e., the typical E. rhusiopathiae sequence, the E. tonsillarum sequence, and a third sequence found for two strains. Strains of the same serotype were found to have different ribopatterns as well as different partial 16S rDNA sequences.
通过用限制性内切酶EcoRI切割染色体DNA，然后与16S rRNA基因的420 bp内部片段杂交，研究了丹毒丝菌菌株16S rRNA基因的限制性片段长度多态性。对32株丹毒丝菌型菌株和参考菌株以及7株田间菌株进行了分类。所有血清型的参考菌株以及猪丹毒丝菌和扁桃体丹毒丝菌的模式菌株均包括在内。观察到9种核糖体图谱。模式A由16株菌株代表，包括血清型1b、2至8、11至13、15至17、19和23的菌株。模式B由2株菌株（血清型1a和9）代表。模式C由5株菌株（血清型5、6和21）代表。模式D由1株血清型4的菌株代表。模式E由血清型2、7、10、20、22、24和25的11株菌株代表。模式F、G、H和I分别由血清型26、2、18和3的单一菌株代表。所有不同的核糖体图谱都有一些共同的条带。模式B、C和D与模式A最相似，而模式F、G、H和I与模式E相似。对9株选定菌株的16S rRNA基因进行部分测序，得到了三种不同的序列，即典型的猪丹毒丝菌序列、扁桃体丹毒丝菌序列以及在两株菌株中发现的第三种序列。发现同一血清型的菌株具有不同的核糖体图谱以及不同的部分16S rDNA序列。
