Motamed K, Bastiani C, Zhang Q, Bailey A, Shen C K
Department of Genetics, University of California, Davis 95616.
Gene. 1993 Jan 30;123(2):235-40. doi: 10.1016/0378-1119(93)90129-q.
The functional interaction between the human epsilon globin promoter and an erythroid-specific transcription enhancer, 5' HS-2, has been analyzed by transient expression assay. While stepwise deletion of DNA sequences between -852 and -122 had only small effects, removal of the CACC box at position -111 greatly decreased epsilon-globin promoter activity, as well as its response to the enhancer function of 5' HS-2 in erythroid cells. Our data demonstrated that the three ubiquitous promoter elements, the CACC, CCAAT, and TATA boxes, of the epsilon-globin-encoding gene together form a minimal promoter that would interact efficiently with 5' HS-2, and that at least the CACC box is an essential functional component of this enhancer-promoter interaction.
通过瞬时表达分析，对人类ε珠蛋白启动子与红系特异性转录增强子5' HS-2之间的功能相互作用进行了研究。虽然逐步缺失-852至-122之间的DNA序列只有微小影响，但去除-111位的CACC盒会大大降低ε珠蛋白启动子活性，以及其在红系细胞中对5' HS-2增强子功能的反应。我们的数据表明，ε珠蛋白编码基因的三个普遍存在的启动子元件，即CACC、CCAAT和TATA盒，共同构成了一个最小启动子，该启动子能与5' HS-2有效相互作用，并且至少CACC盒是这种增强子-启动子相互作用的一个必需功能组件。
