Kumar M C, York M D, Pomeroy B S
Am J Vet Res. 1977 Feb;38(2):255-7.
A microagglutination antigen treated with tetrazolium dye was produced. The culture used was rendered nonmotile by growing in medium containing 1:1,000 concentration of phenol. A trial was conducted by infecting freshly hatched turkey poults with Arizona hinshawii 7:1,7,8. An equal number of poults were kept as negative controls. Starting with 4-week-old poults, blood samples, cloacal swabs, and environmental samples were obtained at 2-week intervals up to 16 weeks of age. The serum samples were tested by using tubeagglutination (TA), serum plate agglutination (SPA), and microagglutination (MA) tests for A hinshawii 7:1,7,8. A field trial was conducted, using known negative and infected turkey flocks, and serum samples were tested by MA, SPA, and TA tests. The results indicated that MA test was far more sensitive and superior to TA and SPA tests in detecting turkeys infected with A hinshawii 7:1,7,8. The field trial confirmed the laboratory trial observations.
制备了一种用四氮唑染料处理过的微量凝集抗原。所使用的培养物通过在含有1:1000浓度苯酚的培养基中生长而使其失去运动性。用亚利桑那希恩肖菌7:1,7,8感染刚孵出的火鸡雏，进行了一项试验。将等量的雏鸡作为阴性对照。从4周龄的雏鸡开始，每隔2周采集一次血样、泄殖腔拭子和环境样本，直至16周龄。对血清样本进行了试管凝集试验（TA）、血清平板凝集试验（SPA）和微量凝集试验（MA），以检测亚利桑那希恩肖菌7:1,7,8。使用已知的阴性和感染火鸡群进行了一项现场试验，并通过MA、SPA和TA试验对血清样本进行检测。结果表明，在检测感染亚利桑那希恩肖菌7:1,7,8的火鸡时，微量凝集试验远比试管凝集试验和血清平板凝集试验敏感且更具优势。现场试验证实了实验室试验的观察结果。
