Bessesen D H, Richards C L, Etienne J, Goers J W, Eckel R H
Laboratoire de Biochimie, Faculte de Medecine St. Antoine, Paris, France.
J Lipid Res. 1993 Feb;34(2):229-38.
Lipoprotein lipase (LPL) is important for the delivery of triglyceride fatty acids (TGFA) to a variety of tissues. We have used measurements of heparin-releasable LPL activity, immunohistochemistry, in situ hybridization, and Northern analysis to more fully characterize the location of LPL within the entire central nervous system (CNS) of the rat. Surprisingly, the levels of LPL activity and mRNA in the caudal spinal cord were 5- to 10-times higher than those found in any other area of the brain, levels similar to those found in adipose tissue or skeletal muscle. A number of cell types including neurons in the hippocampus, Purkinje cells of the cerebellum, and cells deep within the cortex were identified as sources of LPL mRNA. LPL protein was found within many vascular structures throughout the CNS, and within Purkinje cells. The strongest immunostaining was around nerve rootlets associated with the caudal spinal cord. Feeding studies were carried out with [14C]oleic acid to see whether CNS LPL functioned in the uptake of TGFA. These studies demonstrated uptake of chylomicron triglyceride fatty acids throughout the CNS. The localization of LPL within these structures suggests that the uptake of triglyceride fatty acids is an integral part of normal lipid metabolism of the central nervous system and may be important in regulating feeding behavior and/or maintaining normal neuronal function.
脂蛋白脂肪酶（LPL）对于将甘油三酯脂肪酸（TGFA）输送到多种组织至关重要。我们运用肝素可释放LPL活性测定、免疫组织化学、原位杂交和Northern分析等方法，更全面地描绘了LPL在大鼠整个中枢神经系统（CNS）中的定位。令人惊讶的是，尾侧脊髓中LPL活性和mRNA水平比大脑其他任何区域高出5至10倍，与脂肪组织或骨骼肌中的水平相似。包括海马神经元、小脑浦肯野细胞以及皮质深层细胞在内的多种细胞类型被确定为LPL mRNA的来源。在整个中枢神经系统的许多血管结构以及浦肯野细胞中发现了LPL蛋白。最强的免疫染色出现在与尾侧脊髓相关的神经根周围。用[14C]油酸进行喂养研究，以观察中枢神经系统LPL是否在TGFA摄取中发挥作用。这些研究表明，整个中枢神经系统都存在乳糜微粒甘油三酯脂肪酸的摄取。LPL在这些结构中的定位表明，甘油三酯脂肪酸的摄取是中枢神经系统正常脂质代谢的一个组成部分，可能在调节进食行为和/或维持正常神经元功能方面具有重要意义。
