Larson H E, Welch A
Clinical Research Centre, Medical Research Council, Harrow.
J Med Microbiol. 1993 Feb;38(2):103-8. doi: 10.1099/00222615-38-2-103.
In hamsters, resistance to colonisation by Clostridium difficile appears to be mediated by micro-organisms that are present in the gut in relatively low concentrations. Small amounts of normal caecal contents inhibited the growth of C. difficile when added to cultures in vitro or given to animals which had been treated with clindamycin. Filtrates of caecal contents, frozen and thawed contents and contents diluted to 0.1% wet weight lost their inhibitory properties. However, caecal contents retained their protective capacity after culture for 7 days in vitro. Antibiotic treatment altered resistance to colonisation by only a few species of clostridia. Faeces of animals treated with ampicillin but not clindamycin recovered colonisation resistance after incubation at 37 degrees C in vitro. Since human faeces could also restore colonisation resistance to hamsters, the hamster model may be useful for the study of resistance to colonisation by C. difficile in man.
在仓鼠中，对艰难梭菌定殖的抵抗力似乎由肠道中相对低浓度存在的微生物介导。当将少量正常盲肠内容物添加到体外培养物中或给予已用克林霉素治疗的动物时，其可抑制艰难梭菌的生长。盲肠内容物的滤液、冻融后的内容物以及稀释至0.1%湿重的内容物失去了其抑制特性。然而，盲肠内容物在体外培养7天后仍保留其保护能力。抗生素治疗仅改变了少数梭菌属物种对定殖的抵抗力。用氨苄青霉素而非克林霉素治疗的动物粪便在37℃体外孵育后恢复了定殖抵抗力。由于人类粪便也可恢复仓鼠的定殖抵抗力，因此仓鼠模型可能有助于研究人类对艰难梭菌定殖的抵抗力。
