H1受体介导组胺对豚鼠胃平滑肌中肌醇磷酸的反应。

H1 receptor mediates inositol phosphates response to histamine in gastric smooth muscle of guinea pigs.

作者信息

Sim S S, Jo Y H, Hahn S J, Yoon S H, Rhie D J, Kim M S

机构信息

Dept. of Physiology, Catholic University Medical College, Seoul, South Korea.

出版信息

Scand J Gastroenterol. 1993 Jan;28(1):69-72. doi: 10.3109/00365529309096047.

DOI:10.3109/00365529309096047

PMID:8430274

Abstract

The effect of histamine on [3H]-inositol phosphates (IPs) formation was investigated with [3H]-inositol-labeled gastric smooth-muscle cells in guinea pigs. Histamine (10(-5) M) increased the formation of [3H]-IPs in the muscle cells. The increase was significantly inhibited by pyrilamine (10(-5) M) but not by cimetidine (10(-5) M). The contractile response to histamine was also completely inhibited by pyrilamine but not by cimetidine. Phorbol ester 12-myristate 13-acetate (10 microM) significantly inhibited histamine-stimulated [3H]-IPs formation by 56%, whereas forskolin (10 microM) decreased it by 18%. This study demonstrates that the response of [3H]-IPs formation and contraction to histamine is mediated through H1 receptor, and the formation of [3H]-IPs is negatively regulated by protein kinase C in gastric smooth muscle of guinea pigs.

摘要

用[3H]-肌醇标记的豚鼠胃平滑肌细胞研究了组胺对[3H]-肌醇磷酸酯（IPs）形成的影响。组胺（10^(-5)M）增加了肌肉细胞中[3H]-IPs的形成。这种增加被吡苄明（10^(-5)M）显著抑制，但未被西咪替丁（10^(-5)M）抑制。对组胺的收缩反应也被吡苄明完全抑制，但未被西咪替丁抑制。佛波酯12-肉豆蔻酸酯13-乙酸酯（10μM）显著抑制组胺刺激的[3H]-IPs形成达56%，而福斯可林（10μM）使其减少18%。本研究表明，[3H]-IPs形成和收缩对组胺的反应是通过H1受体介导的，并且[3H]-IPs的形成在豚鼠胃平滑肌中受到蛋白激酶C的负调控。