Few J D, Haspineall J R
Ann Clin Biochem. 1977 Jan;14(1):35-8. doi: 10.1177/000456327701400106.
Steady-state gel filtration has been used to study the binding of cortisol to human plasma proteins in vitro. Raising the temperature from 37 degrees C to 41 degrees C results in the mean proportion of free (non-protein-bound) cortisol rising approximately from 7% to 11%. Addition of cortisol to plasma identical to 275 nmol/l) also increased the proportion of free cortisol by approximately 50%. Cortisone is less strongly bound to plasma proteins than cortisol. The mean values (+/- S.D.) for five samples were free cortisol 8.4 +/- 1.1% and free cortisone 26.0 +/- 3.8%.
稳态凝胶过滤已被用于体外研究皮质醇与人血浆蛋白的结合。将温度从37摄氏度升至41摄氏度会导致游离（非蛋白结合）皮质醇的平均比例从约7%升至11%。向血浆中添加等同于275纳摩尔/升的皮质醇也会使游离皮质醇的比例增加约50%。可的松与血浆蛋白的结合比皮质醇弱。五个样本的平均值（±标准差）为游离皮质醇8.4±1.1%，游离可的松26.0±3.8%。
