Burke C W
J Clin Pathol. 1969 Nov;22(6):683-6. doi: 10.1136/jcp.22.6.683.
The determination of the ;cortisol-binding capacity' by gel filtration of a small plasma sample at low temperature after the addition of cortisol results in an arbitrary measurement dependent upon experimental conditions. It is unsatisfactory also because it misleads as to conditions at 37 degrees C. Steady-state gel filtration of cortisol in plasma can be done at 37 degrees C, at which temperature it overcomes known variables in present methods. It should prove of clinical value in showing abnormalities of cortisol binding in disease.
在低温下对添加皮质醇后的少量血浆样本进行凝胶过滤来测定“皮质醇结合能力”，其结果是一种取决于实验条件的任意测量值。它也不令人满意，因为它会误导人们对37摄氏度时情况的判断。血浆中皮质醇的稳态凝胶过滤可在37摄氏度下进行，在此温度下它克服了现有方法中已知的变量。它在显示疾病中皮质醇结合异常方面应具有临床价值。
