Bibikova A, Oron U
Department of Zoology, George S. Wise Faculty of Life Sciences, Tel Aviv University, Ramat Aviv, Israel.
Anat Rec. 1993 Mar;235(3):374-80. doi: 10.1002/ar.1092350306.
The effect of low-energy laser (He-Ne) irradiation on the process of skeletal muscle regeneration after cold injury to the gastrocnemius muscle of the toad (Bufo viridis) was studied using quantitative histological and morphometric methods. The injured zones in the experimental toads were subjected to five direct He-Ne laser (632.8 nm wavelength) irradiations (6.0 mW for 2.3 min) every alternate day starting on the fourth day postinjury. Muscles that were injured as above, and subjected to red-light irradiation, served as a control group. Morphometric analysis was performed on histological sections of injured areas at 9, 14, and 30 days postinjury. At 9 days postinjury, mononucleated cells populated 69.3% +/- 16.8% of the total area of injury. Thereafter, their volume fraction (percent of total injured zone) decreased gradually but more rapidly in the laser-irradiated muscle than in the control. The volume fraction of the myotubes in the laser-irradiated muscles at 9 days of muscle regeneration was significantly higher (7.0% +/- 2.2%) than in the control muscle (1.2% +/- 0.4%). Young myofibers in the laser-irradiated muscles populated 15.5% +/- 7.9% and 65.0% +/- 9.5% of the injured area at 9 and 14 days of muscle regeneration, respectively, while in control muscles these structures were not evident at 9 days and made up only 5.3% +/- 2.9% of the traumatized area at 14 days postinjury. The volume fraction of the young myofibers further increased by 30 days of muscle regeneration making up 75.7% +/- 13.2% of the traumatized area, while in the laser-irradiated muscles most of the injured zone was filled with mature muscle fibers.(ABSTRACT TRUNCATED AT 250 WORDS)
采用定量组织学和形态计量学方法，研究了低能量激光（氦氖）照射对蟾蜍（绿蟾蜍）腓肠肌冷损伤后骨骼肌再生过程的影响。实验蟾蜍的损伤区域在受伤后第4天开始每隔一天接受5次氦氖激光（波长632.8 nm）直接照射（功率6.0 mW，照射2.3分钟）。将上述受伤并接受红光照射的肌肉作为对照组。在受伤后9天、14天和30天，对损伤区域的组织切片进行形态计量分析。受伤后9天，单核细胞占损伤总面积的69.3%±16.8%。此后，它们的体积分数（占总损伤区的百分比）逐渐下降，但激光照射组肌肉中的下降速度比对照组更快。肌肉再生9天时，激光照射组肌肉中肌管的体积分数显著高于对照组（7.0%±2.2%），对照组为（1.2%±0.4%）。肌肉再生9天和14天时，激光照射组肌肉中的年轻肌纤维分别占损伤区域的15.5%±7.9%和65.0%±9.5%，而对照组肌肉在9天时这些结构不明显，受伤后14天时仅占创伤区域的5.3%±2.9%。肌肉再生30天时，年轻肌纤维的体积分数进一步增加，占创伤区域的75.7%±13.2%，而激光照射组肌肉中大部分损伤区域已被成熟肌纤维填满。（摘要截断于250字）
