Wilson Klaire, Terlouw Abby, Roberts Kevin, Wolchok Jeffrey C
Department of Biomedical Engineering, College of Engineering, University of Arkansas, 125 Engineering Hall, Fayetteville, AR, 72701, USA.
Cell and Molecular Biology Program, University of Arkansas, Fayetteville, USA.
J Mater Sci Mater Med. 2016 Aug;27(8):125. doi: 10.1007/s10856-016-5735-0. Epub 2016 Jun 20.
The use of decellularized skeletal muscle (DSM) as a cell substrate and scaffold for the repair of volumetric muscle loss injuries has shown therapeutic promise. The performance of DSM materials motivated our interest in exploring the chemical and physical properties of this promising material. We suggest that these properties could serve as a blueprint for the development of next generation engineered materials with DSM mimetic properties. In this study, whole human lower limb rectus femoris (n = 10) and upper limb supraspinatus muscle samples (n = 10) were collected from both male and female tissue donors. Skeletal muscle samples were decellularized and nine property values, capturing key compositional, architectural, and mechanical properties, were measured and statistically analyzed. Mean values for each property were determined across muscle types and sexes. Additionally, the influence of muscle type (upper vs lower limb) and donor sex (male vs female) on each of the DSM material properties was examined. The data suggests that DSM materials prepared from lower limb rectus femoris samples have an increased modulus and contain a higher collagen content then upper limb supraspinatus muscles. Specifically, lower limb rectus femoris DSM material modulus and collagen content was approximately twice that of lower limb supraspinatus DSM samples. While muscle type did show some influence on material properties, we did not find significant trends related to sex. The material properties reported herein may be used as a blueprint for the data-driven design of next generation engineered scaffolds with muscle mimetic properties, as well as inputs for computational and physical models of skeletal muscle.
将脱细胞骨骼肌（DSM）用作细胞基质和支架来修复大面积肌肉损失损伤已显示出治疗前景。DSM材料的性能激发了我们探索这种有前景材料的化学和物理性质的兴趣。我们认为这些性质可为开发具有DSM模拟特性的下一代工程材料提供蓝图。在本研究中，从男性和女性组织供体中收集了整个人类下肢股直肌样本（n = 10）和上肢冈上肌样本（n = 10）。对骨骼肌样本进行脱细胞处理，并测量和统计分析了九个反映关键组成、结构和力学性质的属性值。确定了每种属性在不同肌肉类型和性别中的平均值。此外，还研究了肌肉类型（上肢与下肢）和供体性别（男性与女性）对每种DSM材料属性的影响。数据表明，由下肢股直肌样本制备的DSM材料具有更高的模量，且胶原蛋白含量高于上肢冈上肌。具体而言，下肢股直肌DSM材料的模量和胶原蛋白含量约为下肢冈上肌DSM样本的两倍。虽然肌肉类型确实对材料属性有一定影响，但我们未发现与性别相关的显著趋势。本文报道的材料属性可作为数据驱动设计具有肌肉模拟特性的下一代工程支架的蓝图，以及骨骼肌计算模型和物理模型的输入参数。
