Wang Q, Ris H B, Altermatt H J, Reynolds B, Stewart J C, Bonnett R, Lim C K
MRC Toxicology Unit, Medical Research Council Laboratory, Carshalton, Surrey, UK.
Biomed Chromatogr. 1993 Jan-Feb;7(1):45-7. doi: 10.1002/bmc.1130070112.
A high performance liquid chromatographic method for the determination of the photodynamic chemotherapeutic agent 5,10,15,20-tetra(m-hydroxyphenyl)chlorin (m-THPC) in human plasma following intravenous infusion is described. The procedure involves extraction of the drug in plasma with methanol/dimethyl sulphoxide (4:1 v/v) containing 5,10,15,20-tetra(p-hydroxyphenyl)chlorin as the internal standard and separation on a C18 reversed phase column with acetonitrile:0.1% trifluoroacetic acid (77:23 v/v) as the mobile phase. The drug was detected specifically and sensitively at its absorption maximum of 423 nm with a detection limit of 15 ng/mL (signal-to-noise ratio of 5). The intra- and inter-assay coefficients of variation (CV) on analysis of a plasma spiked with m-THPC (1 micrograms/mL) were 2.3 and 3.4% (n = 6), respectively.
本文描述了一种高效液相色谱法，用于测定静脉输注后人体血浆中光动力化疗药物5,10,15,20-四（间羟基苯基）二氢卟吩（m-THPC）。该方法包括用含有5,10,15,20-四（对羟基苯基）二氢卟吩作为内标的甲醇/二甲基亚砜（4:1 v/v）从血浆中提取药物，并在C18反相柱上进行分离，流动相为乙腈:0.1%三氟乙酸（77:23 v/v）。在423 nm的最大吸收波长处特异性且灵敏地检测到该药物，检测限为15 ng/mL（信噪比为5）。对添加了m-THPC（1微克/毫升）的血浆进行分析时，批内和批间变异系数（CV）分别为2.3%和3.4%（n = 6）。
